To investigate the intervention effect of electroacupuncture on fear extinction in rats with post-traumatic stress disorder(PTSD)，and its impact on key proteins of the amygdala related to the WW domain-containing E3 ubiquitin protein ligase 2(WWP2)/tumor suppressor phosphatase and tensin homolog(PTEN)/protein kinase B(AKT) signaling pathway，as well as synaptic plasticity proteins synaptophysin(SYN) and growth-associated protein-43(GAP-43).Methods:Male Sprague-Dawley(SD) rats were randomly divided into the blank group，model group，and electroacupuncture group，with 8 rats in each group.PTSD was induced using the enhanced continuous single stress method，followed by electroacupuncture treatment for 21 days.The intervention effect was evaluated using open field test，contextual conditioned fear response experiment，Western blot，co-immunoprecipitation，and immunohistochemistry.Results:Compared to the blank group，the model group exhibited reduced activity distance，fewer central crossings，and fewer standing counts(P<0.05)，confirming successful modeling.Compared to the model group，the electroacupuncture group showed significant improvement in these indicators(P<0.05).During the fear extinction training and fear reinstatement testing phases，the electroacupuncture group exhibited a lower percentage of freezing time than the model group(P<0.05).In the amygdala，the expression of WWP2 and phosphorylated AKT proteins was lower in the model group than that in the blank group(P<0.05)，while PTEN protein expression was higher(P<0.05).These protein expression changes were reversed in the electroacupuncture group(P<0.05)，with an increased binding ability of PTEN to WWP2.Additionally，compared to the blank group，the expression of SYN and GAP-43 proteins in the amygdala was lower in the model group(P<0.05)，while it was higher in the electroacupuncture group(P<0.05).Conclusion:Electroacupuncture effectively improves autonomous activity and exploratory behavior in PTSD rats，promotes the extinction of fear memory，and these effects are related to the regulation of the WWP2/PTEN/AKT signaling pathway in the amygdala and synaptic plasticity.