Drug-induced liver injury(DILI) is a common clinical liver disease.Given the low correlation between existing animal experimental models and clinical symptoms，further research is needed to replicate a DILI model that better reflects the clinical reality.This study aims to analyze and summarize the relevant data of DILI animal models from January 2000 to July 2023，providing references for constructing animal models that are closer to human pathogenesis and liver metabolic functions in clinical practice.Methods:Relevant literature on DILI animal models was searched and selected.A database was created，and the animals used for modeling，modeling methods，modeling duration，positive drugs，and detection indicators were summarized and analyzed.Results:Mice accounted for 74.14% of DILI animal models，with C57BL/6 mice accounting for 27.41%，KM mice for 25.07%，ICR mice for 10.70%，and Balb/c mice for 7.57%.Acetaminophen was the most commonly used drug，accounting for 63.57%.A statistical analysis of the modeling doses for acetaminophen in the included studies showed that the most common dose for mice was 300 mg/kg，accounting for 31.46%，while the most common dose for rats was 1 000 mg/kg.The most common modeling time was 24 h(40%)，followed by 12 h(23.24%) and 6 h(16.22%).The most commonly used administration route was intraperitoneal injection(56.87%)，followed by gavage(41.51%).Common detection methods included Western blot(27.45%) and real-time PCR(26.61%).The main indicators measured were serum glutamic-pyruvic transaminase(GPT)，glutamic-oxaloacetic transaminase(GOT)，alkaline phosphatase(ALP)，and total bilirubin(TBIL).The most common positive control drug was silymarin(37.61%)，followed by N-acetylcysteine(NAC) and biphenyl dimethyl dicarboxylate(26.61% and 27.52%，respectively).By assigning weights to the selected modeling conditions，the optimal model was determined to be male C57BL/6 mice aged 6 to 8 weeks，with a single intraperitoneal injection of acetaminophen at 300 mg/kg.After 24 h，the mice's observable indicators，serum，liver，and other measures should be evaluated to match clinical symptoms，indicating successful model creation.This optimal model accounted for 56.42%.Using male C57BL/6 mice aged 6 to 8 weeks，a single intraperitoneal injection of acetaminophen at 300 mg/kg successfully establishes the DILI model within 24 h.Silymarin，NAC，or biphenyl dimethyl dicarboxylate can be used as positive controls.Detection methods such as Western blot，real-time PCR，and ELISA are employed to assess serum levels of GPT，GOT，ALP，TBIL，and combined liver index and histological results to evaluate model establishment，detection，and evaluation indicators.Conclusion:Through literature mining and systematic analysis，the past patterns and potential issues of DILI model establishment were summarized，and the optimal conditions and evaluation indicators for replicating DILI animal models were identified.This provides a reference for creating DILI animal models with high clinical relevance and reproducibility.