To establish a quantitative analysis of multi-components by a single marker(QAMS) method for the simultaneous determination of nine components in Mongolian medicine Shahuang Qingyi Tablets，including orientin，calycosin-7-O-β-D-glucoside，vitexin，picroside Ⅱ，narcissoside，1,5-dicaffeoylquinic acid，diosmetin，isorhamnetin，and formononetin.Methods:High-performance liquid chromatography(HPLC) was employed using an Agilent Eclipse Plus C18 column(250 mm×4.6 mm，5 μm).The mobile phase consisted of acetonitrile-methanol-0.4% phosphoric acid aqueous solution with a gradient elution at a flow rate of 1.0 mL/min.The column temperature was set at 30 ℃，and the detection wavelength was 245 nm.Using 1,5-dicaffeoylquinic acid as the internal reference，the relative correction factors(fs/i) of orientin，calycosin-7-O-β-D-glucoside，vitexin，picroside Ⅱ，narcissoside，diosmetin，isorhamnetin，and formononetin were determined to quantify these nine components in Shahuang Qingyi Tablets.The stability and robustness of the relative correction factors were evaluated across different chromatographic columns and instruments.The results obtained using the QAMS method were compared with those obtained using the external standard method(ESM).Results:The relative correction factors for the eight components in Shahuang Qingyi Tablets were determined as follows：orientin(1.228 1)，calycosin-7-O-β-D-glucoside(1.129 6)，vitexin(0.750 7)，picroside Ⅱ(0.352 1)，narcissoside(0.880 9)，diosmetin(1.115 3)，isorhamnetin(0.625 1)，and formononetin(4.160 4).The relative standard deviations(RSDs) of the correction factors across three different HPLC instruments and three different C18 columns ranged from 0.76% to 2.78%.Under different column temperatures(25 ℃，30 ℃，35 ℃，40 ℃)，the RSDs ranged from 0.70% to 1.76%，and under different flow rates(0.6，0.8，1.0，and 1.2 mL/min)，the RSDs ranged from 0.34% to 2.08%，indicating good robustness of the relative correction factors.The relative errors(REs) between the QAMS and ESM results were all below 2%，showing no significant difference.Conclusion:The QAMS method using 1,5-dicaffeoylquinic acid as the internal reference is efficient，accurate，and feasible for the determination of nine components in Shahuang Qingyi Tablets.This method can be effectively applied for quality evaluation.