Systems biology，represented by holographic biology，provides a quantitative interpretation of the kidney Zang manifestation theory.Genomics reveals the biological foundation of kidney-stored essence，transcriptomics elucidates the regulatory mechanisms of the kidney governing reproduction-stimulating essence，proteomics clarifies the material basis of the kidney governing original qi，and metabolomics explains the metabolic mechanisms of the kidney governing water.The roles of osteoblasts and osteoclasts help decode the mechanism of the kidney governing bones，while immune cells provide insights into the kidney's role in producing marrow.These interconnected systems collectively construct the life map of the kidney governing the brain.Taking ankylosing spondylitis(AS)—which is rooted in kidney deficiency—as an example，this paper integrates the seven structural systems of kidney Zang manifestation with holographic biology.By exploring the application of the holographic biology framework in AS，this study aims to elucidate the modern scientific connotations of kidney Zang manifestation theory in traditional Chinese medicine(TCM).