To investigate the effect and mechanism of Wenjing Decoction-containing serum on the proliferation，migration，and apoptosis of ectopic endometrial cells.Methods:Female Wistar rats were used to prepare blank serum，Wenjing Decoction-containing serum，and positive drug-containing serum to treat the endometrial epithelial cell line End1/E6E7.Immunofluorescence was used to detect proliferating cell nuclear antigen(PCNA) and Cleaved Caspase-3 expression.Flow cytometry was employed to determine cell cycle and apoptosis.Scratch assay was conducted to determine cell migration.Immunoblotting was applied to determine Cleaved Caspase-3 expression.Fluorescence quantitative PCR was utilized to determine lncRNA-H19 and miR-216a-5p mRNA expression.Dual Luciferase was used to detect the binding relationship between lncRNA-H19 and miR-216a-5p.RNA immunoprecipitation(RIP) assay was performed to observe the binding ability of lncRNA-H19 and miR-216a-5p.Results:Compared with the blank serum group，the cellular activity was decreased in Wenjing Decoction-containing serum and positive drug-containing serum groups(P<0.01)，of which the serum containing 10% of Wenjing Decoction had the best inhibitory effect.After the intervention of Wenjing Decoction-containing serum and positive drug-containing serum，PCNA expression，cell migration rate and miR-216a mRNA expression were decreased in cells，while the cell proportion in G0/G1 phase，Cleaved Caspase-3 expression，apoptosis rate，and lncRNA-H19 mRNA expression were increased，which were significantly different from that in the blank serum group(P<0.01).Dual Luciferase and RIP assays confirmed that lncRNA-H19 was able to bind to miR-216a-5p and inhibit its expression.After LncRNA-H19 overexpression，the lncRNA-H19 mRNA expression level was increased in End1/E6E7 cells treated with Wenjing Decoction-containing serum(P<0.01)，while the miR-216a mRNA expression level was decreased(P<0.01).In addition，lncRNA-H19 overexpression reversed the effects of Wenjing Decoction-containing serum on the proliferation，migration，and apoptosis of End1/E6E7 cells.Conclusion:Wenjing Decoction-containing serum inhibits proliferation and migration and induces apoptosis of endometriotic cells by regulating the lncRNA-H19/miR-216a axis