To preliminarily explore the mechanism by which the Jianpi Xiaoai Prescription reduces the resistance to 5-fluorouracil(5-Fu) in colon cancer.Methods:The 5-Fu-resistant colon cancer cells were transplanted into the axilla of nude mice to establish a drug-resistant tumor model.Once tumors were established，the mice were divided into four groups based on the treatment：model group，5-Fu group，Jianpi Xiaoai Prescription group，and Jianpi Xiaoai Prescription+5-Fu group.The intervention lasted for 5 weeks.The body weight，tumor growth(tumor volume)，and tumor weight of the mice were observed.The expression levels of phosphatase and tensin homolog(PTEN) and Survivin were measured.Results:The body weight of mice in the Jianpi Xiaoai Prescription and Jianpi Xiaoai Prescription+5-Fu groups increased significantly compared with the model and 5-Fu groups(P<0.05).Both the Jianpi Xiaoai Decoction and Jianpi Xiaoai Prescription+5-Fu groups significantly inhibited the growth of drug-resistant transplanted tumors，with statistically significant differences compared with the model and 5-Fu groups(P<0.05).The PTEN protein expression was increased，and Survivin protein expression was decreased in the Jianpi Xiaoai Prescription and Jianpi Xiaoai Prescription+5-Fu groups，with significant differences compared with the model group(P<0.05).Conclusion:Jianpi Xiaoai Prescription combined with chemotherapy can stabilize the body weight of colon cancer model nude mice and inhibit the growth of drug-resistant transplanted tumors.This effect may be related to the upregulation of PTEN protein and downregulation of Survivin protein expression