To establish quality standards for Spatholobi Caulis of gynecological antipruritic tablets.Methods:A thin-layer chromatography method was adopted to conduct qualitative identification on Spatholobi Caulis.The developing agent is a mixture of petroleum ether，dichloromethane，ethyl acetate，and formic acid(7:1:1.6:0.4)，and the chromogenic reagent is a 10% sulfuric acid ethanol solution，with the color developed under a UV lamp(365 nm).Additionally，the high performance liquid chromatography(HPLC) method was employed to determine the content of genistein and formononetin.The dilution method was utilized to determine in vitro antibacterial activity of each medicinal material in gynecological antipruritic tablets.Results:TLC chromatographic spots showed clear color development，and the negative control had no interference.There was a sound linear relationship between the mass concentrations of genistein and formononetin within the range of 12.86 to 1.928 μg/mL and 12.99 to 1.948 μg/mL respectively(r>0.999 0)，with the content of 4.09 and 6.82 mg/g respectively.The results of in vitro antibacterial activity showed that the MIC of the prescription for gynecological antipruritic tablets against Staphylococcus aureus was 27.55 mg/mL and that against Escherichia coli was 13.78 mg/mL.Conclusion:The proposed method is accurate，stable，and feasible，and can be adopted for quality control of gynecological antipruritic tablets(Spatholobi Caulis).