电针刺激对脊髓损伤大鼠神经再生可塑性研究
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国家自然科学基金项目(81660814)——电针通过Nogo-A/NgR与NGF/TrkA的crostalk调节SCI后神经可塑性的机制研究


Effects of Electroacupuncture Stimulation on Nerve Regeneration Plasticity in SCI Rats
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    摘要:

    目的:通过电针刺激脊髓损伤(SCI)大鼠督脉穴、足阳明胃经穴,探讨“同病异治”理论指导下电针刺激对脊髓损伤修复的影响。方法:将120只斯泼累格·多雷(SD)大鼠以随机数字表法随机分为5组:督脉电针组、足阳明胃经电针组、混合电针组、损伤对照组和假手术组,每组24只,每个组再采用随机数字表法随机分为干预前组、治疗后7 d组、14 d组、21 d组,每亚组6只,以脊髓损伤运动功能评价工具BBB评分直观角度观察SCI大鼠下肢运动功能恢复,以免疫组化、透射电镜微观形态下观察细胞形态变化。结果:与损伤对照组比较,足阳明胃经电针组、督脉电针组和混合电针组随着时间的增加,BBB评分均有一定程度的升高,各时间点和损伤对照组比较差异均有统计学意义(均P<0.05),其中混合电针组升高最为明显。随着时间的推移,SCI细胞出血、炎症、脂肪变逐渐减少,SCI细胞形态逐渐修复。结论:不同经络、穴位在不同时间点的电针刺激可改善SCI大鼠BBB评分,减少出血、炎症渗出及脂肪变性,修复细胞形态,促进脊髓神经细胞再生,证明中医“同病异治”理论在神经再生康复方面具有指导意义。

    Abstract:

    To explore the effects of electroacupuncture stimulation on spinal cord injury(SCI) repair under the guidance of the theory of “treating the same disease with different therapies” by electroacupuncture stimulation of the governor vessel acupoint and the foot yang brightness stomach channel acupoint in SCI rats.Methods:A total of 120 Sprague Dawley(SD) rats were randomly divided into five groups:a governor vessel electroacupuncture group,a foot yang brightness stomach channel electroacupuncture group,a mixed electroacupuncture group,an injury control group,and a sham operation group using a random number table,with 24 rats in each group.Then,in the same method,each group was randomly divided into a pre-intervention group,as well as 7-day,14-day,and 21-day after treatment groups,with 6 rats in each subgroup.The motor function recovery of SCI rats' lower limbs was observed from the visual angle of the spinal injury motor function evaluation tool BBB score.Cell morphological changes were observed by immunohistochemistry and the microscopic morphology of the transmission electron microscope.Results:Compared with the injury control group,the BBB scores increased to some extent with the increase of time in the foot yang brightness stomach channel electroacupuncture group,the governor vessel electroacupuncture group,and the mixed electroacupuncture group.Compared with the injury control group,there were significant differences between each time point in the above three groups(P<0.05),especially in the mixed electroacupuncture group.As time passed,the bleeding,inflammation,and steatosis of SCI cells gradually decreased,with the SCI cell morphology being gradually repaired.Conclusion:Electroacupuncture stimulation of different meridians and acupoints at different time points can improve the BBB score of SCI rats,reduce bleeding,inflammatory exudation,and steatosis,as well as repair cell morphology,promoting spinal nerve cell regeneration.Therefore,it is proved that the theory of “treating the same disease with different therapies” in traditional Chinese medicine is of guiding significance in nerve regeneration and rehabilitation.

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易姣君,石鹏,周宾宾.电针刺激对脊髓损伤大鼠神经再生可塑性研究[J].世界中医药,2025,(07).

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  • 收稿日期:2023-12-11
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  • 在线发布日期: 2025-06-11
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