To investigate the effects of Anemarrhenae Rhizoma and its split fractions on the level of material and energy metabolism in normal rats，and to clarify the cold and heat properties and scientific connotation of the property and flavor of traditional Chinese medicine.Methods:According to the random number table method，male SD rats were randomly divided into the blank group，stir-fried Anemarrhenae Rhizoma Decoction group，polysaccharide group，saponin group，ethyl acetate group，and the remaining group，with ten rats in each group.The medicine was given at 9 o'clock a.m.every day.The blank group was given the same volume of physiological saline for gavage administration，with the dosage of 2.16 g/kg for the Anemarrhenae Rhizoma Decoction group，0.26 g/kg for the polysaccharide group，0.13 g/kg for the saponin group，0.026 g/kg for the ethyl acetate group，and 0.09 g/kg for the remaining group，with the medicine given for 21 consecutive days.Additionally，the transcriptomics and proteomics of liver tissue were studied，and bioinformatics analysis was conducted on differentially expressed genes and proteins to find the key differentially expressed genes and proteins related to material and energy metabolism，which were verified by real-time quantitative PCR and Western blotting.Results:The transcriptomics showed that the decoction，polysaccharide，saponin and the remaining fractions could significantly reduce the expression levels of pyruvate kinase(Pklr) and other genes，and inhibit the pyruvate metabolism and PPAR signaling pathway of rats，thereby inhibiting energy metabolism.In contrast，the ethyl acetate fraction showed the opposite trend.Proteomics demonstrated that the decoction，polysaccharide，saponin and the remaining fractions notably decreased the expression levels of Pklr，malate dehydrogenase 2(Mdh2) proteins，and significantly inhibit energy metabolism-related pathways such as the glycolysis and pyruvate metabolism，thus inhibiting energy metabolism.The joint analysis showed that the pyruvate metabolism pathway played an important role in Anemarrhenae Rhizoma and its fractions' part in regulating energy metabolism in rats.Conclusion:The decoction，polysaccharide，saponin and the remaining fractions inhibit energy metabolism of rats，with cold(cool) properties.Additionally，the ethyl acetate fraction promotes the energy metabolism of rats，showing heat(warm) properties.