To explore the pharmacological mechanism of curcumol on glycolysis of hepatic sinusoidal endothelial cells and sinusoidal capillarization.Methods:Hepatic sinusoidal endothelial cells were divided into a blank group，a model group，and curcumol groups(12.5，25，and 50 mg/L).The kit was used to observe the effect of curcumol on the proliferation and migration of hepatic sinusoidal endothelial cells and to detect the effect of curcumol on the glucose uptake，as well as lactic acid and adenosine triphosphate(ATP) production of hepatic sinusoidal endothelial cells.Enzyme-linked immunosorbent assay(ELISA) was conducted to detect the effect of curcumol on the expression and activity of hexokinase 2(HK2)，phosphofructokinase 2(PFK2)，phosphofructokinase 1(PFK1)，pyruvate kinase M2(PKM2)，and lactate dehydrogenase A(LDH-A).An immunofluorescence assay was performed to detect the effect of curcumol on the expression of platelet-endothelial cell adhesion molecule(CD31)，vascular haemophilic factor(vWF)，and collagen IV.Scanning electron microscopy was applied to observe the effect of curcumol on the fenestration on the surface of hepatic sinusoidal endothelial cells.Western blot(WB) and RT-PCR were employed to observe the effect of curcumol on the expression of phosphatidylinositol 3-kinase(PI3K) and protein kinase B(AKT) molecules.Results:Cell Counting Kit-8(CCK8) assay revealed that curcumol inhibited the proliferation of hepatic sinusoidal endothelial cells.The Transwell assay found that curcumol inhibited the migration of hepatic sinusoidal endothelial cells.Kit assay discovered that curcumol decreased glucose uptake，as well as the production of lactic acid and ATP in hepatic sinusoidal endothelial cells.ELISA demonstrated that curcumol inhibited the expression and activity of HK2，PFK1，PKM2，and LDH-A.Immunofluorescence assay manifested that curcumol reduced the expression of CD31，vWF，and Collagen IV.Scanning electron microscopy unveiled that curcumol increased the fenestration on the surface of hepatic sinusoidal endothelial cells.Molecular biology experiments showed that curcumol inhibited the expression of PI3K and AKT.Conclusion:Curcumol inhibits the activity of the PI3K/AKT pathway，glycolysis of hepatic sinusoidal endothelial cells，and sinusoidal capillarization，which may be its anti-hepatic fibrosis mechanism.