To elucidate the function and mechanism of Nao Qingling Tang (Nao Qingling Decoction) for the Treatment of Gilles de la Tourette syndrome.Methods: 84 Kunming mice were randomly divided into 6 groups,namely,the normal control group,model group,positive control group(treated by haloperidol),and Nao Qingling Tang (Nao Qingling Decoction) treated groups of three kinds of dosage,which was 20g/kg,15g/kg and 10g/kg respectively.14 animals in each group.The model of experimental tourette syndrome(ETS) was established by stimulating central nervous system of mice with AP,then the stereotypia and autonomic activities of mice were observed,and fluorophotometry was used to determine the levels of monoamine neurotransmitters such as dopamine(DA),norepinephrine(NE),5 hrdroxytryptamine(5 HT) and 5 hydroxyindoleacetic acid (5 HIAA) in the brains.Results: Compared with that of model group,AP induced stereotypia of mice in Nao Qingling Tang (Nao Qingling Decoction) treated groups and positive control group were obviously relieved(P＜001).Autonomic activities of mice in Nao Qingling Tang (Nao Qingling Decoction) treated groups of dosage of 20g/kg and 15g/kg and positive control group were significantly reduced(P＜001),and that was reduced in mice of Nao Qingling Tang (Nao Qingling Decoction) treated group of dosage of 10g/kg too(P＜005).Moreover,the content of DA and NE in corpora striata of mice in Nao Qingling Tang (Nao Qingling Decoction) treated groups and positive control group were obviously reduced(P＜001).The content of 5 HT and 5 HIAA of mice in Nao Qingling Tang (Nao Qingling Decoction) treated groups of dosage of 20g/kg and 15g/kg were significantly reduced(P＜001),while those in Nao Qingling Tang (Nao Qingling Decoction) treated group of dosage of 10g/kg and positive control group didn’t show significant changes.Conclusion: Nao Qingling Tang (Nao Qingling Decoction) has therapeutical effect on ETS model and its functionary mechanism may be related with its effects on secretion or metabolism of neurotransmitter in the brains.