世界中医药

目的：探讨IgA肾病大鼠肾小球系膜细胞的表型转化及中药复方肾络宁的作用。方法：SD大鼠随机分为正常组、模型组、肾络宁组、肾炎康复片组；采用牛血清白蛋白和葡萄球菌肠毒素B复合感染的方法建立大鼠IgA肾病模型；常规检测尿红细胞、尿蛋白、血肌酐，光镜、电镜、荧光免疫观察肾组织病理；免疫组织化学染色和计算机图象分析系统观察大鼠肾小球系膜细胞α 平滑肌肌动蛋白(α SMA)、纤维连接蛋白(FN)、细胞间黏附分子(ICAM 1)的表达情况。结果：模型组肾小球系膜区α SMA、FN、ICAM 1明显阳性表达(P＜0.01)，系膜区重度IgA沉积，系膜细胞中度增生，基质增多，毛细血管狭窄，血尿及蛋白尿、血肌酐明显增高；肾络宁及肾炎康复片组α SMA、FN、ICAM 1表达明显减低(P＜0.01)，IgA轻度沉积，系膜细胞及基质轻度增多，血尿、蛋白尿及血肌酐明显减低。结论：IgA肾病大鼠肾小球系膜细胞发生表型转化，中药复方肾络宁可明显抑制IgA肾病大鼠肾小球系膜细胞的表型转化，改善临床及病理变化，肾络宁是防治IgA肾病的有效方药。

English Summary:

To investigate transformation of mesangial cell in rats with IgA nephropathy and effect of Chinese materia medica prescription Shenluoning.Methods:SD rats were divided into normal group,model group,Shenluoning group,and Shenyan Kangfu tablet group;The model of IgA nephropathy of rats were established by co infection of bovine serum albumins(BSA) and staphyloentero toxin B (SEB);RBC,BUN,Cr were examined,pathology of nephridial tissue were investigated by optical and electron microscopy and fluorescent immunoassay;Expression of Mesangial Cell α,α SMA,FN,ICAM 1 were observed by Immunohistochemistry and computer image analysis.Results:Expression of Mesangial Cell α,α SMA,FN,ICAM 1 of model group were positive(P<0.01),and in this group,IgA heavily deposit in mesangial region,intercapillary cells moderately proliferated,matrix increased,and blood capillary stenosis could be seen,RBC,BUN,and content of Cr in blood obviously increased;In Shenluoning group and Shenyan Kangfu tablet group,expression of Mesangial Cell α,α SMA,FN,ICAM 1 significantly decreased(P<0.01),deposit of IgA and proliferation of matrix slightly increased,RBC,BUN,content of Cr in blood obviously decreased.Conclusions:Phenotypic transformation of Mesangial cell occur in rats with IgA Nephropathy.Chinese materia medica prescription Shenluoning can repress the transformation,improve clinical and pathological change,Shenluoning is effective in treating IgA Nephropathy.

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