| 引用本文:邓淑渊，余林中.HPLC法测定江南卷柏片中穗花杉双黄酮的含量[J].世界中医药,2008,3(3):. |
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| HPLC法测定江南卷柏片中穗花杉双黄酮的含量 |
| Determination of Amentoflavone in Jiangnan Spikemoss Tablets with HPLC |
| 投稿时间：2008-04-03 |
| DOI： |
| 中文关键词: 江南卷柏片 穗花杉双黄酮 HPLC法测定 |
| English Keywords:Jiangnan Spikemoss Tablets Amentoflavone HPLC determination |
| 基金项目: |
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| 中文摘要: |
| 目的：建立高效液相色谱法测定江南卷柏片中穗花杉双黄酮的含量。方法：C18柱（250mm×4.6mm，5μm）；流动相：乙腈（A）-0.5%冰醋酸溶液（B），梯度洗脱；检测波长：338nm；流速：1ml/min。结果：穗花杉双黄酮在5.25～262.5μg/ml间，峰面积与其质量呈良好的线性关系，回归方程为A=62621C+39.79，r2=1；平均回收率为97.1%，RSD为1.9%。结论：该方法可以用来控制江南卷柏片的质量，方法简便、准确。 |
| English Summary: |
| to establish a HPLC method to determine amentoflavone in Jiangnan Spikemoss Tablets.Methods:detective conditions are as follows: C18 Column (250mm×4.6mm, 5μm); mobile phase: ace(A)-0.5% glacial acetic acid (B), gradient elution; detective wavelength: 338nm; flow rate: 1ml/min.Results:area of the peak showed a good linear relationship with content within the range of 5.25~262.5μg/ml, regression equation: A=62621C+39.79, r2=1 with the average recovery rate being 97.1%, RSD=1.9%.Conclusion:the method is convenient and accurate for determination of amentoflavone in Jiangnan Spikemoss Tablets. |
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