Abstract:to establish a HPLC method to determine amentoflavone in Jiangnan Spikemoss Tablets.Methods:detective conditions are as follows: C18 Column (250mm×4.6mm, 5μm); mobile phase: ace(A)-0.5% glacial acetic acid (B), gradient elution; detective wavelength: 338nm; flow rate: 1ml/min.Results:area of the peak showed a good linear relationship with content within the range of 5.25~262.5μg/ml, regression equation: A=62621C+39.79, r2=1 with the average recovery rate being 97.1%, RSD=1.9%.Conclusion:the method is convenient and accurate for determination of amentoflavone in Jiangnan Spikemoss Tablets.