| 引用本文:蒋秋燕，唐玉云，蒋桂秀.消癥煎膏直肠给药诱导大鼠子宫肌瘤细胞凋亡的研究[J].世界中医药,2008,3(6):. |
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| 消癥煎膏直肠给药诱导大鼠子宫肌瘤细胞凋亡的研究 |
| Study on Hysteromyoma Apoptosis Induced by Mass-Clearing Extraction per Rectum |
| 投稿时间：2007-09-05 |
| DOI： |
| 中文关键词: 子宫肌瘤/中医药疗法 @ 消癥煎膏 |
| English Keywords:Hysteromyoma/ Chinese Medical Therapy @ Mass-Clearing Extraction |
| 基金项目: |
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| 中文摘要: |
| 目的：观察直肠给药（消癥煎膏）对子宫肌瘤模型鼠肌瘤细胞凋亡的干预作用。方法:SD雌性大鼠（正常动物对照组除外）参照Fujii S法，造子宫肌瘤模型，随机分成5组，即动物模型组、消癥煎膏高、中、低剂量组、桂枝茯苓胶囊对照组。放射免疫法测定雌二醇水平，免疫组化法观察病理形态学的变化以及细胞凋亡基因Bcl-2蛋白的表达。结果:高剂量组消癥煎膏能显著抑制模型大鼠瘤样增生(P<0.01)，并降低模型大鼠血液中雌激素的水平(P<0.01)。模型组平滑肌Bcl-2蛋白阳性点数量明显增多，阳性信号显著增强，Bcl-2表达明显增多(P<0.01)。结论:消癥煎膏具有诱导子宫肌瘤细胞凋亡的作用，能降低子宫肌瘤模型鼠细胞凋亡蛋白Bcl-2阳性表达，抑制子宫肌瘤细胞增殖，缩小子宫肌瘤。 |
| English Summary: |
| To observe the intervention of Mass-Clearing Extraction (per rectum) on hysteromyoma apoptosis of rat model.Methods:hysteromyoma models were established on SD female rats according to Fujii S methods，and randomly divided to 5 groups，ie. The model group，high-dosage，middle-level dosage，low-dosage group of the extraction，the control group (administered on Guizhi Fuling Jiaonang，Cinnamon Twig and Poria Capsule). Estradiol levels were detected by radio-immunity method; pathomorphological changes and expression of Bcl-2，a gene of apoptosis were observed by immune histochemistry methods.Results:high dosage of the extraction can inhibit tumor related accrementition (P<0.01)，and bring down estrin level (P<0.01). In the model group there were increased counting of positive cell of Bcl-2，intensified positive signal and more expression of Bcl-2 (P<0.01).Conclusion:Mass-Clearing Extraction can diminish hysteromyoma by inducing apoptosis of hysteromyoma，reducing Bcl-2 positive expression and inhibit hysteromyoma accrementition. |
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