世界中医药

目的：探讨珍珠草乙肝舒康胶囊抗HBV及保护肝细胞的药效学作用及其特点。方法：通过体外2.2.15细胞株实验观察珍珠草乙肝舒康胶囊抑制HBV的效果，建立大鼠CCl₄慢性肝损伤模型，分为空白对照组，CCl₄组，乙肝宁组，珍珠草乙肝舒康大、中小剂量组，检测各组生化及肝左叶病理学组织差异；参照文献方法，测定小鼠腹腔巨噬细胞吞噬功能及小鼠血清溶血素含量，观察珍珠草乙肝舒康对小鼠免疫功能的影响。结果：HBsAg，HBeAg的最大抑制率为76.47%(d7)，71.43%(d5)，且呈现HBV分泌、复制越是旺盛，抑制作用越强的特点；珍珠草乙肝舒康组与模型组相比，肝功能明显改善，肝组织结构破坏轻，纤维组织增生程度轻，与乙肝宁组相比，各项指标变化略占优势；小鼠腹腔巨噬细胞吞噬功能增强，羊红细胞致敏的小鼠血清溶血素含量提高，显著优于空白对照组，与阳性对照组盐酸左旋咪唑片相似。结论：珍珠草乙肝舒康胶囊具有抗HBV、保护肝细胞作用。

English Summary:

to conduct a pharmacodynamic research into HBV recovery capsule prepared with Common Leafflower about its anti-HBV and protective effect of liver cells.Methods:anti- HBV effect was observed through in vitro experiment in 2.2.15 cell line. Rat model of chronic liver injury was established using CCl₄, and was divided to blank control group, CCl₄ group, Yigan Ning Group, and Leafflower high-dose group, middle-dose group and low-dose group. Each group was subject to biochemical examination and histological examination to pathological changes in left hepatic lobe; peritoneal macrophage phagocytosis and content of haemolysin were determined in mice to observe the capsule’s effect on immune function.Results:maximum inhibition ratio of HBsAg and HBeAg were respectively 76.47%(d7) and71.43%(d5), moreover, HBV secretion and replication correlated positively with inhibitive action. When compared with CCl₄ group, the leafflower group improved significantly in liver function, with less severe liver disorganization and fibroplasias; and was superior over various parameter value to Yigan Ning group. The leafflower group had similar effect as Levamisole, which was better than the blank control group: phagocytosis of peritoneal macrophage was reinforced, and content of haemolysin increased following sensitization induced by sheep red blood cell.Conclusion:HBV recovery capsule prepared with Common Leafflower had anti-HBV effect and protection of liver cells.

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