世界中医药

作者	单位
柏冬¹，胡芳²，詹旺达²，彭娟³，范斌³	1 北京中医药大学，北京市北三环东路11号，100029； 2 首都医科大学； 3 中国中医科学院医学实验中心

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中文摘要:

目的：建立测定人参、三七颗粒中四种皂苷类成分含量的高效液相色谱方法。方法：Waters Nova-Pak C₁₈（150×3.9mm 4μm）色谱柱，流动相为乙腈-水溶液梯度洗脱，流速为1.0mL/min，检测波长为203nm。结果：三七皂苷R1、人参皂苷Rg1、人参皂苷Re和人参皂苷Rb1分别在0.210～1.890μg，0.822～7.398μg，0.203～1.827μg及11.22～10.98μg范围内线性关系良好，相关系数r均大于0.9995。三七皂苷R1的回收率为101.7，RSD=2.3%(n=5)；人参皂苷Rg1的回收率为97.4，RSD=1.3%(n=5)；人参皂苷Re的回收率为98.9，RSD=1.6%(n=5)；人参皂苷Rb1的回收率为100.4 RSD=0.9%(n=5)。结论：本方法操作简便，分离效果好，灵敏度高，重复性好，可用于控制人参、三七颗粒的质量。

English Summary:

To establish a method for the determination of the contents of 4 Ginsenosides in granules of ginseng and sanchi.Methods:The determination was carried out with Waters Nova-Pak C18 column (150×3.9mm 4mm）, using acetonitrile -water as the mobile phase (gradient elution) at a flow rate of 1 ml/min and detected at the wavelength 203 nm.Results:The calibration curves of Notoginsenoside R1, Ginsenoside Rg1, Ginsenoside Re, Ginsenoside Rb1 were in good linearity over the range of 0.210-1.890μg, 0.822-7.398μg, 0.203-1.827μg and 11.22-10.98μg, with r=0.9998, 0.9999, 0.9996 and 0.9999, respectively. The average recoveries for Notoginsenoside R1, Ginsenoside Rg1, Ginsenoside Re, Ginsenoside Rb1 were 101.7 with RSD (n=5) 2.3%, 97.4 with RSD (n=5) 1.3%, 98.9 with RSD (n=5) 1.6%, and 100.4 with RSD (n=5) 0.9%, respectively.Conclusion:The method is simple, sensitive, accurate and reproducible, and can be used to control the qualities of granules of ginseng and sanchi.

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