| 引用本文:孔令春，陈志霞，孙玉舫，王松坡.至真方对人大肠癌多药耐药细胞株HCT-8/VCR的增殖抑制及凋亡诱导作用[J].世界中医药,2010,5(4):. |
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| 至真方对人大肠癌多药耐药细胞株HCT-8/VCR的增殖抑制及凋亡诱导作用 |
| Effects of Zhi Zhen Formula on Proliferation Inhibition and Apoptosis Inducing on Human Colorectal Cancer Resistance Cell HCT-8/VCR |
| 投稿时间：2010-03-15 |
| DOI： |
| 中文关键词: 大肠癌/中医药疗法 @ 至真方 |
| English Keywords:Colorectal cancer/ Chinese medical therapy @ Zhi Zhen Formula |
| 基金项目: |
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| 中文摘要: |
| 目的：研究至真方含药血清对人大肠癌多药耐药细胞株HCT-8/VCR的增殖抑制及凋亡诱导作用。方法：用MTT法观察至真方含药血清对HCT-8及其耐药细胞HCT-8/VCR的增殖抑制作用；分光光度法检测至真方含药血清作用HCT-8/VCR细胞后Caspase-3活性；流式细胞术检测至真方含药血清对细胞凋亡率的影响。结果：不同体积分数的至真方含药血清对HCT-8及耐药细胞HCT-8/VCR存在明显的增殖抑制作用（P<0.01），且对两细胞系的抑制无统计学差异(P>0.05)。10%体积分数的至真方含药血清作用HCT-8/VCR细胞24、48、72、96h后Caspase-3活性变化呈时间依赖性，并在72h之后出现OD峰值（OD=1.4，并达到平台。含药血清作用HCT-8/VCR细胞72h后的细胞凋亡率明显高于空白对照组(P<0.01)。结论：至真方含药血清对人大肠癌多药耐药HCT-8/VCR细胞生长有明显增殖抑制作用，在一定条件下呈时间、浓度依赖性，并可诱导HCT-8/VCR细胞凋亡，其机制可能与Caspase-3活性升高有关。 |
| English Summary: |
| To investigate the proliferation inhibition and apoptosis inducing on human colorectal cancer resistance cell HCT-8/VCR by serum containing Zhi Zhen formula.Methods:MTT assay was used to investigate inhibition on cells, flow cytometry to study cell apoptosis, spectrophotometric method to observe Caspase-3 activity.Results:Different volume fractions of drug-serum could inhibit proliferation of HCT-8 and HCT-8/VCR (P<0.01) , having no statistical differences between the two cell lines. Caspase-3 activity was time-dependent based on observation at 24、48、72、96h time points after 10% ( volume fraction) of the serum was given, reaching peak OD value (1.4) after 72 hours when adapoptosis rate of HCT-8/VCR were higher than that of control group (P<0.01). Conclusion:Serum containing Zhi Zhen formula can inhibit the growth of HCT-8 cells and multidrug resistant HCT-8/VCR by inducing apoptosis, which is dose-dependent and time-dependent under certain conditions and may be related to the increase of Caspase-3 activity. |
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