| 引用本文:叶晔，阮善明，沈敏鹤，宋巧玲，陈美雪，林红，任函承.中医补益法优化干预结肠癌辅助治疗阶段的实验研究[J].世界中医药,2011,6(5):. |
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| 中医补益法优化干预结肠癌辅助治疗阶段的实验研究 |
| On TCM Tonification Method as Adjunctive Therapy in Treatment of Colon Cancer |
| 投稿时间：2010-12-10 |
| DOI： |
| 中文关键词: 结肠癌/中医药疗法 补益法 |
| English Keywords:Colon cancer/ Chinese medical therapy Tonification method |
| 基金项目:浙江省中医药青年课题项目（2009YB010）；国家公益性行业科研专项项目（200807018） |
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| 中文摘要: |
| 目的：观察不同时机运用中医补益法协同化疗抗结肠癌的有效性以及可能的作用机制，探索、评估和验证结肠癌辅助治疗阶段中医补益法干预的中西医优化治疗模式。方法： 1）建立人结肠癌LoVo细胞裸鼠移植性肿瘤模型。 2）将60只造模成功裸鼠用随机数字表随机分为6组，提前干预组、同步干预组、延后干预组、化疗组、中药组和模型组，每组10只。 3）细胞移植后第8天开始按治疗方案干预不同的实验分组，中医补益法采用灌胃的方法分组给药，5-Fu化疗采用腹腔注射给药。每天观察瘤块生长、摄食、摄水和活动状态等情况。 4）用药结束，摘眼球法处死各组一半裸鼠，剥取瘤块，测量体积，称取瘤重，免疫组化分析凋亡相关蛋白Bcl-2、Bax的表达，其余观察生存期。结果： 1）平均肿瘤体积：除中药组外，其余不同药物干预组均与模型组比较有统计学意义（P<0.05），且延后干预组与化疗组比较也有统计学意义（P<0.05）。 2）平均瘤重：除中药组、化疗组外，其余不同药物干预组的瘤重均与模型组比较有统计学意义（P<0.05），且延后干预组与化疗组比较也有统计学意义（P<0.05）。 3）抑瘤率：中药组1.89%，化疗组7.55%，同步干预组13.21%，提前干预组13.21%，延后干预组18.87%。 4）平均生存期：除中药组与化疗组外，其余不同药物干预组的生存期均与模型组比较有统计学意义（P<0.05），且提前干预组、延后干预组与化疗组比较也有统计学意义（P<0.05）。 5）Bcl-2的平均光密度：提前干预组、延后干预组均与模型组比较有统计学意义（P<0.05），延后干预组与化疗组比较也有统计学意义（P<0.05）。 6）Bax的平均光密度：除中药组和化疗组外，其余不同药物干预组均与模型组比较有统计学意义（P<0.05），延后干预组与化疗组比较有统计学意义（P<0.05）。结论：中医补益法协同化疗治疗LoVo人结肠癌移植性裸鼠的生存期比化疗组有所延长，延后干预组和提前干预组较之同步干预组有更大的生存获益趋势，延后干预组较之提前干预组有更大的生存获益趋势；中医补益法协同化疗的作用机制可能与凋亡蛋白Bcl-2和Bax的表达有关。 |
| English Summary: |
| To observe the clinical effectiveness and mechanism of TCM Tonification Method combined with chemotherapy for colon cancer treatment during different stages, and verify an optimized TCM Tonification regimen.Methods:We injected human LoVo cell tumor xenografts into immunodeficiency mice, randomized 60 mice into 6 groups: early intervention group, Simultaneous intervention group, delayed intervention group, chemotherapy group, Chinese herbal medicine group and model group. From the 8th day, the groups received respective treatments. Growth of tumor, food intake, water intake and movements of mice were observed every day. At end of treatment, the expression of Bcl-2 and Bax, and survival time were observed.Results:①average size of tumor: Except the Chinese herbal medicine group, there was significantly reduction of tumor size (P<0.05), and the delayed intervention group and the chemotherapy group were also statistically different (P<0.05). ②average tumor weight: Except the Chinese herbal medicine group and chemotherapy group, the tumor weight in various groups were statistically less than model group (P<0.05), and the delayed intervention group and chemotherapy group had statistically differences (P<0.05).③Tumor inhibition rate: 1.89% in Chinese herbal medicine group, 7.55% in chemotherapy group, 13.21% in simultaneous intervention group, 13.21% in early intervention group,18.87% in delayed intervention group.④The average survival time: Except Chinese herbal medicine group and chemotherapy group, the other groups had significantly longer survival time than that of model group (P<0.05), and there was significant difference between early intervention group and chemotherapy group, between delayed intervention group and chemotherapy group (P<0.05).⑤the average optical density of Bcl-2: There were significantly difference between the early intervention group and model group，between delayed intervention group and model group (P<0.05), and also between delayed intervention group and the chemotherapy group, (P<0.05).⑥The average optical density of Bax: Except Chinese herbal medicine group and chemotherapy group, the other different drugs intervention group and model group were significantly different (P<0.05).Conclusion:Delayed intervention group has a greater survival benefit than early intervention group. The mechanism may be associated with expression of apoptotic protein Bcl-2, Bax . |
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