| 引用本文:张万龙 张树峰 范洪亮 沙洁津 于海荣 刘振岳.泽泻汤加味方对高盐饮食高血压大鼠降压作用及机理的实验研究[J].世界中医药,2012,7(6):. |
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| 泽泻汤加味方对高盐饮食高血压大鼠降压作用及机理的实验研究 |
| Mechanism of Zexie Decoction on Hypertension Due to High Salt Diet in Rat |
| 投稿时间：2012-05-24 |
| DOI： |
| 中文关键词: 高血压/中医药疗法 @ 泽泻汤加味方； 动物实验 |
| English Keywords:Hypertension/Chinese medical therapy @ Zexie Decoction Animal experiment |
| 基金项目:河北省科学技术支撑项目计划课题(编号：09276101D-20) |
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| 中文摘要: |
| 目的:探讨泽泻汤加味方降压作用及机制。方法:泽泻汤加味方水煎浓缩剂，分高、中、低剂量组灌胃干预高盐高血压大鼠。设置正常空白组、模型空白组、复代文对照组，测定尿量、血压；28天后，经大鼠腹主动脉动取血，分别采用放射免疫分析法、酶联免疫吸附法、自动生化分析法，测定大鼠血浆AngⅡ、血清EO、ALD含量及红细胞膜Na+,K+-ATPase活性。结果:给药后第1、4、7天，各给药组较模型空白组尿量增加（复代文组＞中药高剂量组＞中药中剂量组＞中药低剂量组）（P<0.01或P<0.05）；中药各组尿量第4天达高峰，至第7天开始减少；1周后，中药各组尿量仍较模型空白组为多，但无统计学意义（P>0.05）；除空白组外，各模型组血压均下降（复代文组＞中药高剂量组＞中药中剂量组＞中药低剂量组＞模型对照组）。各中药组血压值与复代文组比较均有统计学意义（P<0.05或P<0.01），与模型对照组比较亦均有统计学意义（P<0.01或P<0.05）；给药各组的降压作用与其利尿作用（复代文组＞高剂量组＞中剂量组＞低剂量组）呈一致性；1周后，除复代文组的尿量与给药前比较有统计学意义外，中药复方各组并不增加，但降压作用依然存在。各给药组血浆AngⅡ、血清EO、ALD含量不同程度下降，Na+,K+-ATPase活性显著提高。结论:泽泻汤加味方具有一定的降压作用，呈剂量依赖关系。初期的降压作用显著，与其利尿作用有关，长期的降压作用是通过利水、活血、祛痰的综合作用实现的。降压机制与其降低循环AngⅡ、ALD、EO及提高细胞膜Na+,K+-ATPase活性相关。 |
| English Summary: |
| To discuss anti-hypertension effect of Zexie Decoction and its mechanism . Methods:Zexie Decoction (high, medium, and low dose) was given to rats on hyper-salt diet and with hypertension, there were also normal blank group, Model blank group, and Diovan control group. urine volume and blood pressure were measured; 28 days later plasma AngⅡ, sera EO, ALD content and red blood cell membrane Na+, K+ATPase activity were measured using radioimmuno-assay enzyme linked immunosorbent assay, automatism biochemical methods. Results:After drug delivery 1, 4, 7 day, each drug delivery group had urine volume increased than model blank group ( diovan group > Zexie high doses group > Zexie medium group > Zexie low dose group) ( P<0.01 or P<0.05); each Zexie group reached highest urine volume at day 4, and decreased from day7; after 1 week, each Zexie group had more urine volume than model blank group ( P> 0.05); each model group had decreased blood pressure ( diovan group > Zexie high doses group > Zexie medium dose group > Zexie low dose group > model blank control group) .All Zexie groups had significantly different blood pressure than that of the diovan group and model blank control group ( P<0.05 or P<0.01); the anti hypertension effect is consistent with urination promoting effect ( diovan group > high doses group > medium dose group > low dose low dose group); after 1 week, Zexie didn’t increase urine volume, but still with anti-hypertension effect .Each drug delivery group presented decreased plasma Ang Ⅱ, sera EO, ALD content, and increased Na+, K+- ATPase activity.Conclusion:Zexie Decoction has dose-dependant anti-hypertension effect. The initial effect is obvious and related to urine promotion, long-term effect is achieved via diuresis, blood activation, and phlegm resolving.This is achieved through inhibition of Ang Ⅱ, ALD, EO and increase of Na+,K+-ATPase activity of cell membrane. |
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