To explore the effect of Liushen Pill on expression of PDGF and VEGF on H22 hepatoma ascites transplantable tumors. Methods: Models of H22 hepatoma ascites transplantable tumor were established for different groups of intervention. Immunohistochemical method was used to measure the expression of PDGF and VEGF for statistic analysis. Results: the average grey level and positive rate of area density in Liushen Pill group，cisplatin group and combination group were all significantly lower than that of the control group (P<0.01). The lowest grey level and most obvious inhibition effect in combination group showed that combined application of Liushen Pill and cisplatin would bring synergy. Conclusions: Liushen Pill would restrain the generation of tumor blood vessels whose mechanism shows relevance with inhibition of the expression of PDGF and VEGF.