世界中医药

目的：观察气滞血瘀证大鼠一氧化氮/一氧化氮合成酶（NO/NOS）的体系变化。方法：将Wistar大鼠随机分成4组，对照组、模型组、血府逐瘀汤低、高剂量组。采用多因素联合刺激法建立气滞血瘀模型。运用分光光度法测定血浆和肝组织中NO含量以及肝组织中NOS活性，利用RT-PCR法测定肝组织中NOS基因表达。结果：模型组血浆NO含量（0.52±0.33）μmol/L显著低于对照组（2.77±1.73）μmol/L（P<0.01），低剂量组NO含量（2.37±0.53）μmol/L显著高于模型组（0.52±0.33）μmol/L（P<0.01）；模型组肝组织NO含量（0.52±0.24）μmol/mg显著低于对照组（5.87±1.72）μmol/mg（P<0.01），低剂量组NO含量（3.72±1.53）μmol/mg显著高于模型组（0.52±0.24）μmol/mg（P<0.01）；模型组肝组织eNOS基因表达水平显著低于对照组（P<0.01），低剂量组肝组织eNOS显著高于模型组（P<0.05）。结论：气滞血瘀证大鼠NO/NOS体系下调，为进一步研究治疗血瘀症类疾病提供了借鉴和思路。

English Summary:

To investigate change of nitric oxide（NO）/NO synthase（NOS）in Qi-blood stagnation of rats. Methods:Wistar rats were randomized to four groups: normal control group， model group， low concentration group， and high concentration group. The Qi-blood stagnation model was established by complex pathogens. The content of NO in plasma and liver tissues of rats was measured by spectrophotometric. RT-PCR was used to analyze liver tissues NOS mRNA expression. Results:The plasma NO of model group［（0.52±0.33）μmol/L］ decreased significantly compared with normal control group［（2.77±1.73）μmol/L］（P<0.01）.The plasma NO of treatment group［（2.37±0.53）μmol/L］ increased significantly compared with model group［（0.52±0.33）μmol/L］（P<0.01）.The NO in liver tissues of model group［（0.52±0.24）μmol/mg］ decreased significantly compared with normal control group［（5.87±1.72）μmol/mg］（P<0.01）.The NO in liver tissues of treatment group［（3.72±1.53）μmol/mg］ increased significantly compared with model group［（0.52±0.24）μmol/mg］（P<0.01）.The expression of NOS in liver tissues NOS mRNA expression in model group decreased significantly compared with normal control group (P<0.01). The expression of NOS in liver tissues NOS mRNA expression in low concentration increased significantly compared with model group (P<0.05). Conclusion:Nitric oxide（NO）/NO synthase（NOS）was down-regulated in Qi-blood stagnation rats.

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