引用本文:黄慧莲 刘丽莎 刘荣华 邵 峰 任 刚 舒积成 林 涛.用高效液相色谱法鉴别小叶菝葜[J].世界中医药,2013,8(1):. |
|
用高效液相色谱法鉴别小叶菝葜 |
The Identification of Smilax Microphylla by HPLC |
投稿时间:2012-07-30 |
DOI:10.3969/j.issn.1673-7202.2013.01.035 |
中文关键词: 菝葜科 HPLC 小叶菝葜 |
English Keywords:Smilacacea HPLC Smilax microphylla |
基金项目:国家自然科学基金课题(编号:30960494和编号:81160507);江西省青年科学家培养对象(编号:20112BCB23012);江西省自然科学基金课题(编号:2009GZY0099) |
|
摘要点击次数: 1421 |
全文下载次数: 1252 |
中文摘要: |
目的:利用高效液相色谱法鉴别4种不同产地的小叶菝葜。方法:利用HPLC方法,Thermon C18分析柱(250 mm×4.6 mm,5 μm);柱温为35℃;流动相为乙腈-0.2%磷酸水梯度洗脱;流速1.0mL·min-1;检测波长330nm。结果:小叶菝葜药材色谱图中有18个分离度较好的共有峰。结论:该方法可为小叶菝葜的鉴别研究提供参考。 |
English Summary: |
To identify four kinds of Smilax microphylla produced in different places with the method of HPLC. Methods: The HPLC analysis was performed on a Thermon C18(150mm×4.6mm,5μm)column with gradient mobile phase of acetonitrile-0.2% phosphoric acid at 35℃,the detection wavelength was set at 330 nm with the flow rate of 1 mL?min-1. Results: Eighteen main characteristic peaks in Smilax microphylla were selected. Conclusions:The method can be used to provide the reference for the identification of Smilax microphylla. |
查看全文 查看/发表评论 下载PDF阅读器 |