世界中医药

目的：从NF-κB信号传导通路角度探讨三妙丸类方及引经药川牛膝对急性痛风性关节炎大鼠炎症反应的作用及机制。方法：70只大鼠随机取10只作为正常组，其余60只大鼠制备急性痛风性关节炎大鼠模型，随机分为模型组、二妙丸组、三妙丸低剂量组、三妙丸高剂量组、川牛膝低剂量组、川牛膝高剂量组。观察各组大鼠踝关节周径的变化；药物干预3天后，取大鼠滑膜组织，运用ELISA法、Western blot法测定细胞因子水平及NF-κB P65蛋白表达水平；对滑膜组织进行HE染色，观察其病理学变化。结果：各药物干预组大鼠滑膜组织NF-κB P65、TNF-α、IL-6、IL-8、滑膜组织病理学积分低于模型组（P<0.01，P<0.05）。结论：三妙丸类方及川牛膝通过下调NF-κB P65蛋白表达，抑制TNF-α、IL-6、IL-8的水平，减轻急性痛风性关节炎大鼠踝关节的炎症反应，以三妙丸高剂量组疗效尤为显著；三妙丸抗炎作用优于二妙丸，机制在于引经药川牛膝对二妙丸的药效协同作用。

English Summary:

To research the mechanism of Sanmiao Pill and Medicinal Cyathula root for inflammatory reaction of acute gouty arthritis in rats from the perspective of NF-κB signal conduction. Methods:Ten normal rats as the normal group, randomized other 60 rats of acute gouty arthritis to model group, Ermiao pills group, Sanmiao pill low dose group, Sanmiao pill high dose group, Cyathula low dose group and Cyathula high dose group. Right ankle joint was examined in each group. Measured the cell genes and NF-κB P65 protein expression level by ELISA assay immunohistochemistry and Western blot assay 3 days ofter intervention. Colored synovial tissue by HE staining and observed histopathological changes. Results: The medicine intervention groups' NF-κB P65 expression level in synovial tissue, the level of TNF-α IL-6 IL-8 and The total score of synovial tissue histopathology were significantly lower than that of the model group (P<0.01 or P<0.05). Conclusion: Sanmiao pill and Medicinal cyathula root maintained the level of cell genes TNF-α, IL-6, IL-8 by decreasing NF-κB P65 protein expression, and reduced the inflammatory reaction of acute gouty arthritis in the rats. Sanmiao pill high dose group was most effective, and Sanmiao pill was more effective than Ermiao pill. Medicinal cyathula root plays a role in targeting Ermiao pill at infected site.

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