| 引用本文:王晓娟 孙兆林 季宇彬 李志雄.栀子中两种活性成分的含量测定[J].世界中医药,2013,8(2):. |
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| 栀子中两种活性成分的含量测定 |
| Content Determination of Two Active Ingredients in Fructus Gardeniae |
| 投稿时间：2012-07-20 |
| DOI：10.3969/j.issn.1673-7202.2013.02.030 |
| 中文关键词: 栀子 栀子苷 去乙酰车叶草苷酸甲酯 高效液相色谱法 |
| English Keywords:Fructus Gardeniae Geniposide Deacetyl asperulosidic methyl ester HPLC |
| 基金项目:基金项目：国家科技重大专项《重大新药创制》课题资助（编号：2009ZX09301-001） |
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| 中文摘要: |
| 目的：建立同时测定栀子中去乙酰车叶草苷酸甲酯、栀子苷含量的HPLC分析方法。方法：色谱柱：Agilent Eclipse XDB-C18(4.6 mm×250 mm,5 μm)；流动相：乙腈-0.1%磷酸水溶液，梯度洗脱。结果：去乙酰车叶草苷酸甲酯、栀子苷分别在0.009～0.186mg/mL(r=0.9 994)、0.042～0.846 mg/mL(r=0.9996)浓度范围内与峰面积呈良好的线性关系，平均回收率分别为97.08%，RSD=1.48%和100.45%，RSD=1.29%(n=6)。结论：所建立方法简单、准确，易操作，具有良好的精密度、重复性和回收率，可作为栀子中这两种成分的含量测定方法。 |
| English Summary: |
| To establish a method using HPLC for the simultaneous content determination of geniposide and deacetyl asperulosidic methyl ester in Fructus Gardeniae. Methods:The HPLC method was applied with Agilent Eclipse XDB-C18 column, The mobile phases consisted of acetonitrile and 0.1% phosphoric acid aqueous solution, gradient elution, the detection wave length was at 238 nm and the flow rate was 1ml/min. Results: Deacetyl asperulosidic methyl ester and geniposide showed a good linear relationship in the range of 0.009-0.186mg/ml(r=0.9994),0.042-0.846mg/ml(r=0.9996) respectively, the average recovery were 97.08% and 100.45% with RSD at 1.48% and1.29% (n=6). Conclusion: The method is simple, accurate and easy to operate, has good precision, repeatability and recovery. It can be used to determine contents of these two ingredients in Fructus Gardeniae. |
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