To observe the effects of drug containing serum of Zishentongluo Decoction(ZSTL) on glomerular mesangial cell (GMC) proliferationon in vitro under high glucose to explore its significance in the prevention and treatment of diabetic nephropathy (DN).Methods:The thiazolyl blue tetrazolium bromide (MTT) colorimetric method was used to observe the glomerular mesangial cell proliferation in different time.Results:Compared with the normal group, vitro cultured glomerular mesangial cells obviously proliferated under the stimulation of high glucose and LPS. Compared with the model group, the drug containing serum of Zishentongluo Decoction(ZSTL) could inhibit proliferation of GMCs.Conclusion:Vitro cultured glomerular mesangial cells could obviously proliferate under the stimulation of high glucose and LPS, and drug containing serum of Zishentongluo Decoction(ZSTL) can inhibit the proliferation of GMCs which could be helpful for preventing the progression of diabetic nephropathy.