| 引用本文:丁如贤 李 霞 李一珺 孙雪妹 杨明华.黄芪药材、饮片中黄芪甲苷含量测定方法的改进[J].世界中医药,2013,8(06):. |
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| 黄芪药材、饮片中黄芪甲苷含量测定方法的改进 |
| Improved Method for Determination of Astragaloside in Astragali Radix |
| 投稿时间：2012-11-26 |
| DOI：10.3969/j.issn.1673-7202.2013.06.028 |
| 中文关键词: 黄芪 黄芪甲苷 含量测定 高效液相蒸发光检测 |
| English Keywords:Astragali Radix Astragaloside Determination HPLC-ELSD |
| 基金项目: |
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| 中文摘要: |
| 目的：建立操作简便、重现性好的黄芪药材、饮片中黄芪甲苷的提取及含量测定方法。方法：比较4种不同的提取方法，用HPLC-ELSD法测定黄芪甲苷含量，色谱柱为Capcell PAK C18(4.6 mm×250 mm，5 μm)；流动相为乙腈-水(32∶68)。结果：简化的提取方法，黄芪甲苷的进样量在0.53~12.72 μg范围内线性关系良好(r＝0.999 4)，平均回收率为98.91%，RSD=2.10%。结论：改进方法样品提取简单、方便、准确、重现性好，可作为黄芪药材、饮片中黄芪甲苷含量测定方法。 |
| English Summary: |
| To establish a simple and reproducible method for determination of astragaloside in Astragali Radix.Methods:Four extraction methods were used and compared. The content of astragaloside was determined by HPLC-ELSD with a Capcell PAK C18(4.6 mm×250 mm，5 um)and a mobile phase of acetonitrile -water(32:68).Results:Using the simple extraction method， the linear relation was good with the range of 0.53~12.72 ug(r=0.999 4).The average recovery was 98.91% with RSD=2.10%.Conclusion:The improved method is simple， accurate， and reproducible and can be used for the determination of astragaloside in Astragali Radix. |
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