| 引用本文:施金凤1 于 河2 王 上3 谷晓红2.食积因素对FM1流感病毒感染小鼠免疫功能影响的实验研究[J].世界中医药,2013,8(09):. |
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| 食积因素对FM1流感病毒感染小鼠免疫功能影响的实验研究 |
| Experimental Study of the Impact of Dyspepsia on Immune Function on Influenza Virus Infected Mice |
| 投稿时间：2012-12-27 |
| DOI：10.3969/j.issn.1673-7202.2013.09.029 |
| 中文关键词: 食积 流感病毒 免疫功能 |
| English Keywords:Dyspepsia Influenza Virus Immune function |
| 基金项目:科学研究与研究生培养共建项目——科研项目——应用队列研究序贯扎根理论探索中医病因学研究的新模式(编号：521/0101204);北京市教委共建项目资助(编号：0101165);北京中医药大学自主课题(编号：2009JYBZZ-JS008);北京中医药大学青年教师专项课题：食积与肺肠损伤的相关性实验研究 |
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| 中文摘要: |
| 目的：通过检测不同时相食积复合流感病毒(FM1)感染小鼠血清免疫球蛋白M(IgM)、IFN-γ、IL-4及TH1/TH2水平,探讨食积对流感病毒感染(FM1)小鼠免疫功能的影响。方法：通过喂饲小鼠高蛋白、高热量特制饲料复制食积模型,在此基础上,经鼻感染FM1甲型流感病毒,复制病毒性感染模型,于感染后第1、3、5天,分别检测各组小鼠血清IgM、IFN-γ、IL-4及TH1/TH2水平。结果：小鼠感染FM1流感病毒后第5天,单纯感染组、食积感染组小鼠血清IgM含量均较正常组下降,且食积感染组结果差异有统计学意义(P<0.05),食积感染组较单纯感染组正常组血清IgM含量明显降低,且结果有统计学意义(P<0.01);病毒感染后第1、3、5天,单纯感染组、食积感染组小鼠血清TH1(IFN-γ)/TH2(IL-4))水平均较正常组降低,且在第3天差异有统计学意义(P<0.05),第3、5天食积感染组较正常组降低更为明显,且差异有统计学意义(P<0.05)。结论：食积可能通过影响机体IgM、IFN-γ、IL-4及TH1/TH2水平对流感病毒感染(FM1)小鼠的免疫失衡产生影响,从而提示食积因素可能对流感的发生发展产生了影响。 |
| English Summary: |
| To investigate the impact of dyspepsia on immune function on influenza virus ( FM1 ) infected mice, through detecting the serum levels of immunoglobulin M (IgM), Interferon-γ(IFN-γ), Interleukin-4(IL-4)and TH1/TH2. Methods:The mice dyspepsia model was built by feeding mice with high protein- calorie food, then nasal infected with FM1 influenza A virus, randomized to normal group, infected group, and infected and dyspepsia group on D3 and D5 after infection, detected the serum level of IgM, IFN- γ, IL-4 and TH1/TH2 levels. Results:Mice were infected with FM1 virus at D5, the IgM level of both infected groups were significantly higher than that of the normal group (P<0.05). The dyspepsia group showed significantly decreased IgM level (P<0.01) compared with the virus infected group; at D1, D3, D5, the serum level of TH1 ( IFN-γ) /TH2 ( IL-4 ) of the infected groups were lower than that of the normal group, and the reduction was siginificant on D3 (P<0.05); at D3 and D5 the dyspepsia group was even more lower (P<0.05). Conclusion: Dyspepsia can break the balance of immune system by impacting IgM, IFN- γ, IL-4 and TH1/TH2, indicating dyspepsia plays a role in influenza epidemic. |
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