世界中医药

目的:观察不同方法对腓肠肌急性钝挫伤组织修复的影响,为肌肉损伤的临床治疗提供理论依据。方法:采用定量负荷自由落体打击法复制骨骼肌急性钝挫伤模型。造模成功后将大鼠随机分为6组,即刻予以不同方法干预,观测各组大鼠小腿周径变化;IL-1、IL-8、TNF含量变化;电镜观察肌原纤维损伤情况。结果:电镜下针刺组改变显著,可见肌原纤维损伤部位聚集较多线粒体和肌质网,正常的肌节附近有普遍增多的线粒体,同样范围内肌卫星细胞较其他组多3~4倍;IL-1、TNF水平明显增高的是正红花油组,其次是针刺组和冰敷组,2组间无显著差异;IL-8水平明显增高的是正红花组、针刺组、模型组。结论:骨骼肌损伤后需要良性修复,在临床治疗中,不要一味的消肿、消炎,应考虑如何用药,使肌肉损伤处良性修复,非瘢痕修复,促进肌肉的再生,功能的恢复。

English Summary:

To compare the different emergency treatment to acute closed skeletal gastrocnemius muscle injury of rats, and provides theoretical basis to clinical therapeutics on muscle injury. Methods:Modeling gastrocnemius muscle in rats' hindquarter by using method of free falling with quantitative load to simulate acute skeletal muscle injury. Rats are divided into 6 groups and intervened in acute stage with different methods. After acute stage, we took out gastrocnemius of affected side to measure calf circumference, observed under electron microscope, and performed ELISA analysis. Results: Significant change was seen in the acupuncture group under electron microscopic observation, around myofibrillar injury site there were more mitochondria and sarcoplasmic reticulum, and more mitochondria around normal sarcomere. There was three to four times more muscle satellite cells in the same range than were in other groups. Using Zhenghonghua oil can cause IL-1&TNF level to increase most significantly. The acupuncture & ice groups had IL-1&TNF level increased without significant difference. IL-8 level increased in Zhenghonghua oil, acupuncture and model groups. Conclusion: The skeletal muscle injury need benign repair. Clinical treatment should not focus only on anti-swelling or anti-inflammation medications, but also medication that work to make muscle injury repair benignly and scarlessly, promoting muscle regeneration and functional recovery.

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