| 引用本文:孟红旭 姚明江 刘建勋.人参皂苷Re对大鼠心室肌细胞钠、钾离子通道的影响[J].世界中医药,2013,8(10):. |
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| 人参皂苷Re对大鼠心室肌细胞钠、钾离子通道的影响 |
| Effects of Ginsenoside Re on Sodium and Potassium Current in Rat Ventricular Myocytes |
| 投稿时间：2013-09-12 |
| DOI：10.3969/j.issn.1673-7202.2013.10.005 |
| 中文关键词: 人参皂苷Re 膜片钳 钠通道电流 钾通道电流 |
| English Keywords:Ginsenoside Re Pathch clamp technique Sodium current Potassium current |
| 基金项目:中国中医科学院自助选题项目（编号：zz070821），科技部十二五重大新药创制课题（编号：2012ZX09301002-004） |
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| 中文摘要: |
| 目的：观察人参皂苷Re对大鼠心室肌细胞电压依赖性的钠、钾离子通道的影响，揭示其抗心律失常的作用机制。方法：采用酶分法获得单个大鼠心室肌细胞，在全细胞膜片钳技术模式下，记录电压依赖性钠通道、瞬时外向钾通道和内向整流钾通道电流，并观察不同浓度的人参皂苷Re对电流幅度的影响。结果：给予人参皂苷Re(10,100 μmol·L-1)后钠通道电流幅度呈现减小的趋势，对通道电流幅度的抑制率分别为(25.5±5.7)%（n=3）和(46.2±18)%（n=4）。给予人参皂苷Re(10,100μmol·L-1)对瞬时外向钾电流幅度略有抑制，洗脱后电流恢复，其中100μmol·L-1人参皂苷对电流的抑制率为(13±9)%（n=3）。给予人参皂苷Re(10,100μmol·L-1)后内向整流钾电流幅度呈现逐渐减小的趋势，药物洗脱后电流完全恢复，（10,100μmol·L-1）对通道电流幅度的抑制率分别为(16.3±8.7)%（n=5）和(35.4±11.2)%（n=5）。结论：人参皂苷Re能够抑制心室肌细胞电压依赖性的钠通道、瞬时外向钾通道和内向整流钾通道电流发挥抗心律失常的作用。 |
| English Summary: |
| To investigate the effects of Ginsenoside Re on voltage-dependent sodium current and potassium current in rat ventricular myocytes, and to investigate the mechanism of antiarrhythmia . Methods: The effect of Ginsenoside Re on sodium current(INa), transient outward potassium current(Ito) and inwardly rectifie- d potassium current（Ik1）were assessed by applying whole-cell patch clamp technique in single cardiac myocytes. Results: Ginsenoside Re (10 ,100μmol·L-1)) was shown to inhibit the altitude of INa and the inhibition ratio was 25.5±5.7%（n=3）and 46.2±18%（n=4）. It was shown a little inhibition by Ginsenoside Re (100μmol·L-1)) and its effects were reversible completely after washout. The inhibition ratio of Ito was 13±9%（n=3）in 100μmol·L-1). Ginsenoside Re (10 ,100μmol·L-1)) inhibited the altitude of Ik1 and the inhibition ratio was 16.3±8.7%（n=5）and 35.4±11.2%（n=5）. Conclusion: Ginsenoside Re can inhibit sodium current and potassium currents to produce antiarrhythmic effect. |
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