| 引用本文:王 辉1，2 杜欣颖1，2，3 花宝金1，2 孙桂芝1，2.益气活血法抗乳腺癌EMT6细胞增殖的荷瘤小鼠体内筛方研究[J].世界中医药,2013,8(11):. |
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| 益气活血法抗乳腺癌EMT6细胞增殖的荷瘤小鼠体内筛方研究 |
| A split-side Research of Qi Boosting and Blood Quicken Formula’s Function in Inhibiting the Proliferation of Breast Cancer Cell EMT6 in a Tumor Burdened Mice Model in Vivo |
| 投稿时间：2013-10-30 |
| DOI：10.3969/j.issn.1673-7202.2013.11.022 |
| 中文关键词: 益气 活血 乳腺癌 增殖 |
| English Keywords:Yiqi Huoxue Breast cancer Proliferation |
| 基金项目:国家自然科学基金（编号：81072970） |
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| 中文摘要: |
| 目的：筛选抗乳腺癌增殖的益气药、活血药适宜配伍。探讨益气药、活血药抗肿瘤的配伍的内在含义。方法：建立小鼠乳腺癌EMT6细胞BALB/C小鼠荷瘤模型。按照处理方式设立：空白对照组（Control）；紫杉醇组（Taxel）；黄芪+苏木组（HQ+SM）；黄芪+苏木+蒲黄组（HQ+SM+PH）；黄芪+苏木+蜂房组（HQ+SM+FF）；黄芪+苏木+炮山甲组（HQ+SM+PSJ）；黄芪+苏木+蒲黄+蜂房组（HQ+SM+PH+FF）；黄芪+苏木+蒲黄+炮山甲组（HQ+SM+PH+PSJ）；黄芪+苏木+蜂房+炮山甲组（HQ+SM+FF+PSJ）；黄芪+苏木+蒲黄+蜂房+炮山甲组（HQ+SM+PH+FF+PSJ），共10组。成瘤小鼠共100只，随机分入以上各组中，每组10只。分别给予生理盐水、中药组方灌胃，生理盐水、紫杉醇注射液腹腔注射。观察成瘤后第8天，荷瘤重量。并计算抑瘤率。结果：黄芪+苏木+蜂房+炮山甲组肿瘤组织块重量明显小于空白对照组，差异具有统计学意义，P=0.049<0.05，该组抑瘤率为27.05%。结论：益气药、活血药组合黄芪+苏木+蜂房+炮山甲，具有抑制乳腺肿瘤EMT6细胞在BABL/C小鼠体内增值的作用。具有进一步研究价值。 |
| English Summary: |
| To get effective compatibility of Chinese medicine herbs which would boost qi and quicken blood for inhibiting proliferation of breast cancer cells. And also to explore the connotation of herbs compatibility to treat cancer. Methods: Breast cancer cell EMT6 burdened BALB/C mice model were established.Ten groups were made including control group (Control), Taxel group (Taxel), Huangqi + Sumu group (HQ+SM); Huangqi + Sumu + Puhuang group (HQ+SM+PH); Huangqi + Sumu + Fengfang (HQ+SM+FF); Huangqi + Sumu + Paoshanjia (HQ+SM+ PSJ); Huangqi + Sumu + Puhuang + Fengfang (HQ+SM+PH+FF); Huangqi + Sumu + Puhuang + Paoshanjia (HQ+SM+PH+PSJ); Huangqi + Sumu + Fengfang + Paoshanjia (HQ+SM+FF+PSJ); Huangqi + Sumu + Fengfang + Fengfang + Paoshanjia (HQ+SM+PH+FF+PSJ). 100 tumor burden mice were randomly divided into these ten groups with ten mice in each group. Chinese medicine formula and saline were administered intragastrically to different groups as well as saline and Taxel were given by interoperatoneal injection. The burdened tumor weight was measured 8 days after forming the tumors and the tumor inhibition rate was observed. Results: The weight of burden tumor of the Huangqi+Sumu+Fengfang+Paoshanjia group (HQ+SM+FF+PSJ) was much smaller than that of the control group. The difference was statistically significant, P=0.049<0.05. The tumor inhibition rat of (HQ+SM+FF+PSJ) group was 27.05%. Conclusion: The Chinese medicine herbs compatibility with Huangqi, Sumu, Fengfang and Paoshanjia has the function of inhibiting the proliferation of breast cancer cell EMT6 in BALB/C mice in vivo. |
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