| 引用本文:王鹏军1 孙明谦2 苗 阳2 刘玲玲2.缺血性心力衰竭气虚血瘀证和阳虚水停证的代谢组学研究[J].世界中医药,2013,8(12):. |
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| 缺血性心力衰竭气虚血瘀证和阳虚水停证的代谢组学研究 |
| Metabolomics-Based Study on Ischemia Heart Failure With Syndrome Of Blood Stasis Caused By Qi Deficiency And Syndrome Of Fluid Accumulation Caused By Yang Deficiency |
| 投稿时间：2013-11-07 |
| DOI：10.3969/j.issn.1673-7202.2013.12.004 |
| 中文关键词: 缺血性心力衰竭 气虚血瘀证 阳虚水停证 代谢组学 |
| English Keywords:Ischemia heart failure Syndrome of blood stasis caused by qi deficiency Syndrome of fluid accumulation caused by Yang Deficiency Metabolomics |
| 基金项目:国家自然科学基金资助项目（编号：81072930） |
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| 中文摘要: |
| 目的：研究缺血性心力衰竭气虚血瘀证和阳虚水停证的血浆代谢组学特征，探索代谢组学在缺血性心力衰竭中医证候中的应用。方法：收集缺血性心力衰竭气虚血瘀证和阳虚水停证患者各18例，利用液相色谱质谱（LC/MS）联用技术对样本进行检测，用主成分分析（PCA）及偏最小二乘分析判别法（PLS-DA）处理图谱数据，研究两组血浆代谢物图谱的差异。结果：两组患者血浆LC-MS图谱的PCA及PLS-DA分析显示，气虚血瘀证和阳虚水停证能够被明显区分，两组血浆代谢物含量存在明显差异。缺血性心力衰竭阳虚水停证较气虚血瘀证，3种溶血磷脂酰胆碱类物质含量降低，3种肉碱类物质含量升高，2中脂肪酸类物质含量升高，肌酐含量升高。结论：缺血性心力衰竭阳虚水停证和气虚血瘀证具有不同的代谢模式，两组能量代谢及磷脂代谢存在差异。以LC-MS为技术特点的代谢组学研究方法能够将两者区分，为中医证候的客观诊断提供了新的检测方法。 |
| English Summary: |
| To investigate metabolomics characteristics of serum samples in ischemia heart failure with syndrome of blood stasis caused by qi deficiency (QDBS) and syndrome of fluid accumulation caused by Yang Deficiency (YDFA) and explore the application of metabolomics in study of TCM syndrome of ischemia heart failure. Methods：The serum samples of ischemia heart failure with QDBS (n=18) and YDFA (n=18) were detected by Liquid chromatography- mass spectrometry(LC-MS) and analyzed based on Principal Component Analysis(PCA) and Partial Least Squares Discriminant Analysis(PLS-DA). Results：Ischemia heart failure with QDBS and YDFA can be distinguished clearly by PLS-DA. There was significant difference in metabolic characteristic between the two groups. Compared with QDBS，3 metabolites belonged to Lysophosphatidylcholines decreased in YDFA; 3 metabolites belonged to carnitines and 2 metabolites belonged to fatty and creatinine increased in YDFA. Conclusion：Energy metabolism and phospholipid metabolism were different between ischemia heart failure with QDBS and YDFA. Metabolomics analysis based on LC/MS can distinguish the two groups and provide new detective method for TCM syndromes differentiation objective. |
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