世界中医药

作者	单位
李晋玉1 徐林1 金合2 谭荣伟3，4 俞兴1	1 北京中医药大学东直门医院骨科中心，北京，100700 2 北京市延庆中医院骨科，北京，102100 3 清华大学材料学院，北京，100086 4 深圳兰度生物材料有限公司，深圳，518057

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中文摘要:

目的：本实验采用可注射骨修复材料(Injectable Bone Regeneration Composite，IBRC)复合脂肪间充质干细胞(Adipose Tissue-Derived Mesenchymal Stromal cells，ADMSCs)应用于兔腰椎横突间融合模型，骨碎补总黄酮(Assemble Flavone of Drynaria Rhizome，AFDR)灌胃，观察融合效果。方法：60只成年白兔，制成兔腰椎横突间融合模型，采用安全随机法随机分为5组：A组植入IBRC材料结合AFDR灌胃;B组植入IBRC材料复合自体髂骨(Autologous iliac Crest Bone，ACB)，结合去离子水﹙Deionized Water，DW﹚灌胃;C组植入IBRC材料+ADMSCs，DW灌胃;D组，植入ACB，DW灌胃;E组植入ACB，AFDR灌胃。以术后第8周为观察时间点，进行大体观测，手触检测，X线片，组织学观察。结果：IRBC+ADMSCs，ACB+AFDR组获得较好的融合率，显著高于单纯IRBC，ACB组(P<0.05)，与ACB+DW组相比差异无统计学意义(P>0.05)。结论：自体ADMSCs结合IRBC材料可实现与ACB组相似的融合率，结合中药AFDR灌胃，能够提高兔腰椎横突间的骨性融合率。

English Summary:

To explore the effect of Adipose Tissue-Derived Mesenchymal Stromal cells (ADMSCs) which is Injectable Bone Regeneration Composite (IBRC) combined with intra-gastric administration of Assemble Flavone of Drynaria Rhizome(AFDR) on rabbits with lumbar transverse interbody fusion. Methods：Sixty adult rabbits were made into rabbit lumbar transverse interbody fusion models，randomly divided into five groups including A group (IBRC+AFDR)，B group (IBRC+ Autologous iliac Crest Bone(ACB) + Deionized Water (DW))，C group (IBRC+ADMSCs+DW)，D group (ACB+DW)，E group (ACB +AFDR). After eight weeks of treatment，specimens were collected for gross observation，touch detection，X-ray，histological observation. Results：The fusion rates of group C and group E were significantly higher than that of group B (P<0.05)，and there was no significantly statistical difference (P>0.05)compared with the group D. Conclusion：IRBC composite ADMSCs can achieve a similar fusion rate as ACB group. Combing with traditional Chinese AFDR can improve the rate of the intertransverse lumbar fusion in rabbits.

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