| 引用本文:王佳雯 陆金根 曹永清 潘一滨.姜露散对大鼠感染性创面提脓祛腐的作用及机制[J].世界中医药,2013,8(12):. |
|
| 姜露散对大鼠感染性创面提脓祛腐的作用及机制 |
| Effect and Mechanism of Jianglu Powder in Absorbing Pus and Removing Slough on Infected Wound Surface of Rats |
| 投稿时间：2013-10-08 |
| DOI：10.3969/j.issn.1673-7202.2013.12.028 |
| 中文关键词: 姜露散 感染性创面 提脓祛腐 创面修复 溶菌酶 IL-1β 升丹制剂 |
| English Keywords:Jianglu powder Infected wound surface Absorbing pus and removing slough Wound healing Macrophages lysosome IL-1β Shengdan preparation |
| 基金项目:上海高校青年教师培养资助计划(编号：shzy023);国家中医药管理局重点学科-肛肠学科资助;海派中医流派传承研究基地-顾氏外科(编号：ZYSNXD-CC-APGC-JD002);上海市科委科研计划项目(编号：13401903001) |
|
| 摘要点击次数: 1599 |
| 全文下载次数: 1661 |
| 中文摘要: |
| 目的：研究姜露散对大鼠感染性创面提脓祛腐的作用及机制。方法：制备大鼠背部感染性创面模型。将大鼠随机分为姜露散组、九一丹组、模型组。每天分别给予姜露散、九一丹创面换药，模型组给予生理盐水创面换药，1次/d，连续治疗14 d。观察大鼠一般情况及创面脓腐脱落时间。第3、7、14 d，记录创面分泌物量;免疫组化法检测创面肉芽组织中溶菌酶含量;ELISA法检测创面肉芽组织中IL-1β含量。第7、14 d，酶法检测主要肝肾功能指标。结果：姜露散组和九一丹组创面脓腐脱落时间均明显短于模型组(P<0.01)。姜露散组和九一丹组第3 d创面分泌物量计分均明显高于模型组(P<0.01)。第3、7 d，姜露散组和九一丹组创面肉芽组织中溶菌酶含量均明显高于模型组(P<0.01)，创面肉芽组织中IL-1β含量均明显高于模型组(P<0.01)。第7、14 d，九一丹组血清BUN、CREA、ALT含量明显高于姜露散组和模型组(P<0.05)。结论：姜露散可能通过提高创面肉芽组织中吞噬细胞溶菌酶含量及创面肉芽组织中IL-1β含量来达到对感染性创面提脓祛腐的作用，其用药安全性优于升丹制剂(九一丹)。 |
| English Summary: |
| To explore the effect and mechanism of Jianglu powder (JLP) in absorbing pus and removing slough on infected wound surface of rats. Methods：Rat models of infected wound surface on the back were successfully established. The model rats were randomly divided into the JLP group，the Jiuyi Powder (JYP) group and the model group. The wound surfaces of rats in JLP group were treated with JLP，while those in JYP group were given JYP every day. The wound surfaces of rats in model group were only treated with normal saline (NS) every day. The treatment lasted for fourteen days. The rats’ general situation and the dropping off time of pus and slough were observed. On the 3rd，7th，and 14th day，the amount of wound surface secretion was calculated. The level of macrophages lysosome in wound tissues was detected by immunohistochemistry; the content of IL-1β in wound tissues was detected by ELISA. On the 7th and14th day，serum liver and renal function indexes were detected by enzymic method. Results：In terms of the dropping off time of pus and slough，JLP group and JYP group had significantly shorter results compared with the model group (P<0.01). As for score of wound surface secretion，on the 3rd day，JLP group and JYP group showed significantly higher outcomes than model group(P<0.01).In terms of the level of macrophages lysosome in wound tissues，on the 3rd and 7th day，JLP group and JYP group had significantly higher scores than the model group(P<0.01).The content of IL-1β in wound tissues on the 3rd and 7th day in JLP group and JYP group were significantly more than those in model group(P<0.01). Serum total BUN，CREA and ALT：on the 7th and 14th day in JYP group was significantly higher than JLP group and model group(P<0.05).Conclusion：JLP could absorb pus and remove slough of infected wound surface by increasing the level of macrophages lysosome and IL-1β in wound tissues. The safety of JLP is better than Shengdan preparation (JYP). |
| 查看全文 查看/发表评论 下载PDF阅读器 |
