| 引用本文:周 倩 姚广涛 金若敏 谢家骏.商陆皂苷甲致肾细胞毒性的研究[J].世界中医药,2014,9(02):. |
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| 商陆皂苷甲致肾细胞毒性的研究 |
| Study on Renal Cell Toxicity Induced by Esculentoside A |
| 投稿时间：2014-01-06 |
| DOI：10.3969/j.issn.1673-7202.2014.02.006 |
| 中文关键词: 商陆皂苷甲 HK-2细胞 肾细胞毒性 |
| English Keywords:Esculentoside A HK-2 cells Renal cell toxicity |
| 基金项目:国家重点基础研究发展计划(973计划)项目(编号：2009CB522807);国家“十二五”科技重大专项课题“中药安全评价技术平台”(编号：2011ZX09301-009) |
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| 中文摘要: |
| 目的：考察商陆皂苷甲Esculentoside A(EsA)对人肾小管上皮细胞(HK-2细胞)的毒性。方法：1)MTT法测细胞活力；2)检测细胞上清液LDH、细胞内SOD及MDA；3)采用倒置显微镜及电镜观察细胞形态学；4)采用Hoechst-PI染色及流式细胞仪观察细胞凋亡的情况。结果：1)EsA对肾细胞的活力有显著的抑制作用，其IC50为149.11 μg·mL-1，且存在一定的时效和量效关系；2)EsA能升高肾细胞培养上清中的LDH，使肾细胞内SOD/MDA比值有所下降；3)EsA能使肾细胞及其超微结构发生变化，出现凋亡或坏死，有一定的量效关系。结论：大于100 μg·mL-1浓度的商陆皂苷甲对HK-2细胞有一定的毒性，其毒性机制与细胞氧化应激、凋亡等相关。 |
| English Summary: |
| To investigate the toxicity of Esculentoside A on HK-2 cells (Human renal tubular epithelial cells).Methods：1)MTT assay was used to test the cell viability.2)The LDH in cells supernatant, SOD and MDA in the cells were detected. 3)Morphological changes of the cells were observed through inverted phase contrast microscope and electron microscopy. 4)Cell apoptosis was detected by Hochest33342-PI dyeing and flow cytometry.Results：1)Esculentoside A could inhibit nephrocyte viability dependently on time and dose (IC50 was 149.11μg·mL-1). 2)Esculentoside A could increase the activity of LDH in nephrocyte supernatant, reduce SOD activity and increase MDA content in nephrocytes. 3)Esculentoside A could cause damage of nephrocyte structure, cell apoptosis and necrosis.Conclusion：It showed that large dose of Esculentoside A (more than 100 μg·mL-1 ) has toxicity on HK-2 cells and the mechanisms of toxicity may include cellular oxidative damage and cell apoptosis. |
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