世界中医药

目的：探寻电针对缺血性脑卒中再灌注损害大鼠cAMP反应原件结合蛋白(cAMP-response Element Binding Protein，CREB)表达的影响。方法：将60只健康成年的SD大鼠驯养3 d随机分成假手术组、模型组以及电针组，每组各20只，使用线栓法大脑中动脉闭塞(Middle Cerebral Artery Occlusion，MCAO)对模型组、电针组大鼠进行脑缺血模型制作，缺血2 h后进行再灌注，电针组大鼠接受电针干预，再灌注3 d后将大鼠断头取脑，进行2，3，5-氯化三苯基四氮唑(2，3，5-triphenyltetrazolium chloride，TTC)染色以及图像软件分析观察脑梗死体积，免疫蛋白印迹法(Western blotting)检测CREB蛋白水平，聚合酶链反应(Reverse Transcription-polymerase Chain Reaction，PCR)检测CREB的基因表达水平。结果：TTC及其图像软件分析表明，电针组的SD大鼠脑梗死面积明显小于模型组(P<0.01);电针组的SD大鼠CREB蛋白水平明显高于模型组(P<0.01);电针组的SD大鼠CREB基因水平明显高于模型组(P<0.01)。结论：电针改善脑缺血再灌注损伤可以通过上调CREB实现。

English Summary:

To explore the impact of electro-acupuncture on the expression of CREB in the ischemia-reperfusion rats. Methods： Sixty healthy adult SD rats which were domesticated for 3 days were randomly divided into the shame group, the model group and the electro-acupuncture group. There were 20 rats in each group. The rats of the model group and the electro-acupuncture group were given the line bolt method (MCAO) to complete the brain ischemia model, and were reperfused 2 hours after ischemia. The electro-acupuncture group adopted the intervention of electro-acupuncture. Three days after the reperfusion, all the rats were beheaded and taken brains. The cerebral infarction volume was observed through TTC staining and image analysis, and the protein expression of CREB were detected by protein imprinting method (Western blotting) and gene expression of CREB were tested by Real-Time PCR. Results： TTC and the image analysis showed that the cerebral infarction volume of electro-acupuncture group was significantly smaller than that of the model group (P<0.01); the CREB protein level in the electro-acupuncture group was obviously higher than that of the model group (P<0.01); the CREB gene level in the electro-acupuncture group was much higher than that of the model group (P<0.01). Conclusion： Electro-acupuncture can realize the improvement of the cerebral ischemia reperfusion injury by up-regulating CREB.

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