| 引用本文:郭志丽1 朱 妍1，2 肖红斌3 王 忠1.丹红注射液调节大脑中动脉阻塞大鼠血清氨基酸代谢谱的实验研究[J].世界中医药,2014,9(03):. |
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| 丹红注射液调节大脑中动脉阻塞大鼠血清氨基酸代谢谱的实验研究 |
| Research on Danhong Injection Adjusting Metabolic Spectrum of Serum Amino Acid in Cerebral Infarction Rats |
| 投稿时间：2013-07-22 |
| DOI：10.3969/j.issn.1673-7202.2014.03.031 |
| 中文关键词: 高效液相色谱 氨基酸代谢组学 脑梗死 丝氨酸代谢 |
| English Keywords:HPLC Amino acid Cerebral infarction Serine metabolism Metabolomics |
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| 中文摘要: |
| 目的：利用代谢组学手段检测大脑中动脉阻塞（Middle Cerebral Artery Occlusion,MCAO）模型大鼠血清中氨基酸含量，微观上阐明氨基酸注射液的疗效以及部分作用机制。方法：采用邻苯二甲醛(O-Phthalaldehyde，OPA)柱前在线衍生反相高效液相色谱法，采用荧光检测，梯度洗脱，建立了血清中氨基酸代谢谱的相对定量分析方法，测定36例大鼠血清中的15种氨基酸含量。流动相A为10 mol/L Na2HP04-Na2B07缓冲液(pH=7.95)，B为乙腈-甲醇-水(45：45：15，V/V/V)，线性梯度洗脱，流动相B在6 min内由5%增加至17.2%，6~35 min内由17.2%增加至52%。结果：1)该法在35 min之内分离出15种氨基酸;2)经主成分分析，3个组在空间上得到很好地分离，氨基酸代谢谱发生了一定的变化;3)利用SPSS方差分析，有6个氨基酸在分离各组有统计学意义。结论：丹红注射液通过部分调节MCAO摄动通路改善脑梗死大鼠血清部分氨基酸的代谢趋势。 |
| English Summary: |
| To determine the content of amino acid to clarify the curative effect and the functioning mechanism of DanHong injection on microscopic.Methods：OPA reversed phase high performance liquid chromatography (HPLC)，precolumn derivatization method fluorescence detection， gradient elution were used.Mobile phase A was 15mol/L Na2HP04 - Na2B07 buffer (pH = 7.95)， B was acetonitrile - methanol - water (45：45：15， V/V/V).As for linear gradient elution， mobile phase B in 6 min increased from 5% to 17.2%， increased from 17.2% to 52% in 6-35 min.Results：1) Fifteen kinds of amino acids were isolated within 35 min; 2) using principal component analysis， three groups were well separated; 3) using the SPSS analysis of variance， there were six amino acids in the separation between groups was statistically significant.Conclusion：Danhong injection can improve metabolic trend of amino acid in cerebral infarction rats serum through adjusting perturbed pathways |
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