| 引用本文:刘恩顺1 孙增涛1 苏景深1 杨英伟2 李 建2 李美凤2.ARDS“肺肠同病”的病理特征与通腑泻肺中药作用机制的实验研究[J].世界中医药,2014,9(04):. |
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| ARDS“肺肠同病”的病理特征与通腑泻肺中药作用机制的实验研究 |
| Experimental Study on the Pathological Features of ARDS “Lung and Large Intestine Concurrent Disease” and the Mechanism of Tongfu Xiefei Herbs |
| 投稿时间：2014-03-11 |
| DOI：10.3969/j.issn.1673-7202.2014.04.005 |
| 中文关键词: ARDS 肺肠同病 病理特征 通腑泻肺 作用机制 |
| English Keywords:ARDS Lung and Large Intestine Pathological features Tongfu Xiefei Mechanism |
| 基金项目:国家重点基础研究发展计划(973计划)项目(编号：2009CB522702) |
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| 中文摘要: |
| 目的：在“肺与大肠相表里”理论指导下，观察ARDS“肺肠同病”的病理特征及通腑泻肺中药的作用机制，为“肺肠同病”的临床治疗提供实验依据。方法：健康wistar大鼠30只，随机分为3组，正常组、模型组和治疗组，采用尾静脉注射油酸加内毒素方法造成ARDS模型，治疗组给予通腑泻肺中药，于实验第7天检测分析各组大鼠肺功能、肺脏局部及全身炎症因子、肠黏膜屏障功能及肺肠组织黏膜免疫等指标。结果：1）与正常组比较，模型组大鼠肺顺应性显著降低、吸气和呼气阻力显著升高（P<0.01），治疗后肺顺应性、吸气阻力有所改善。2）模型组大鼠血浆D-乳酸、DAO含量较正常组明显升高（P<0.01）。治疗后二者含量显著降低（P<0.01）。3）模型组大鼠血清及BALF中TNF-α、IL-1β、IL-6、IL-10含量较正常组均明显升高（P<0.01）。治疗后血清TNF-α、IL-1β含量明显降低（P<0.01），BALF中TNF-α、IL-1β和IL-6含量明显降低（P<0.01）。4）模型组大鼠肺与肠组织SIgA含量较正常组明显升高（P<0.01），治疗后显著降低（P<0.05）。结论：1）肺通气功能障碍、肠黏膜屏障功能受损、全身及肺脏局部炎症反应水平明显升高、肺肠组织黏膜免疫的同步反应是ARDS“肺肠同病”的主要病理特征；2）通腑泻肺中药具有肯定的疗效，其作用机制在于改善肺通气和肠黏膜屏障功能、调节炎性因子表达及肺肠黏膜免疫应答。 |
| English Summary: |
| According to the interior-exteriorly related theory of lung and the large intestine, to observe the pathological features of ARDS lung and large intestine concurrent disease, and investigate the mechanism of Tongfu Xiefei herbs in ARDS rats, then provide experimental basis for clinical treatment. Methods： Thirty healthy Wistar rats were randomly divided into 3 groups, control group, model group, and treatment group. The ARDS rats were established by intravenous injecting oleic acid plus endotoxin in caudal vein, and rats in treatment group were treated with Tongfu Xiefei herbs. The lung function, inflammatory cytokines in the lung and serum, intestinal barrier function and the indicators of lung and intestinal mucosal immunity in each group were observed after 7 days. Results：1）Compared with control group, the lung compliance of model group was obviously decreased; the aspiratory and expiratory resistance were obviously increased(P<0.01).The lung function of treatment group improved. 2）The D-Lactate, DAO of model group were significantly increased than those of control group (P<0.01). Both of them decreased significantly after treatment(P<0.01).3）The TNF-α,IL-1β,IL-6,IL-10 in BALF and serum of model group were obviously increased than those of control group(P<0.01). After treatment, TNF-α,IL-1βin serum were significantly decreased(P<0.01).TNF-α,IL-1βand IL-6 in BALF were significantly decreased（P<0.01）.4）The SIgA expression in lung and large intestine of model group were obviously increased than those of control group(P<0.01),and it significantly decreased after treatment(P<0.05).Conclusion： 1）The pathological features of ARDS is lung and large intestine concurrent disease, which mainly include ventilation dysfunction, intestine barrier functional disturbance, the body’s and lung’s inflammation levels significant increase and the synchronous response in lung and intestinal mucosal immune.2）Tongfu Xiefei herbs shows positive curative effect, for they could improve the pulmonary ventilation and intestinal barrier function, regulate inflammatory cytokines expression and lung and intestinal mucosal immune response. |
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