To study LPS signal path changes of lung and large intestine. Methods： Mice were randomly divided into physiological group, hyperoxia group, hypoxia group, diet restriction group, water restriction group. The LPS signal path’s correlation of lung and large intestine was observed detecting changes of variation of TLR4, MD2 and CD14 in lung and large intestine. Results： When TLR4 and MD2 in the lungs of hyperoxia group and hypoxia group increased, they also increased in the large intestines. When TLR4 and MD2 in the large intestines of diet restriction group and water restriction group increased, they also increased in the lungs. Conclusion： Lung and large intestine can be thought to have related synergy identification in TLR4 and MD2 of LPS signal path.