| 引用本文:黄 欣1 李一鸣2 徐福琴1 何明钎1 邴飞虹2.蜈蚣三七纳米粉预防大鼠术后肠粘连的机制研究[J].世界中医药,2014,9(04):. |
|
| 蜈蚣三七纳米粉预防大鼠术后肠粘连的机制研究 |
| Study on Mechanism of Nano-powder of Wugongsanqi in Preventing Postoperative Intestinal Adhesion in Rats |
| 投稿时间：2013-10-21 |
| DOI：10.3969/j.issn.1673-7202.2014.04.023 |
| 中文关键词: 蜈蚣三七 肠粘连 TNF-α IL-1β IL-6 |
| English Keywords:Anemone flaccida Intestinal adhesion TNF-α IL-1β IL-6 |
| 基金项目:湖北省教育厅科研计划重点项目(编号：D20101802);深圳市科技局项目(编号：201103249) |
|
| 摘要点击次数: 1595 |
| 全文下载次数: 1850 |
| 中文摘要: |
| 目的：探讨蜈蚣三七纳米粉预防大鼠术后肠粘连的作用机制。方法：Ellis法建立大鼠术后肠粘连模型,随机分为假手术组、模型组、阳性组(肌内注射地塞米松10 mg/kg)、蜈蚣三七高、中、低剂量组(分别灌胃450、225、112 mg/kg)。术前3 d开始给药,术后继续给药1周。末次给药后,取血清,ELISA法检测TNF-α、IL-1β和IL-6的表达,并进行粘连程度肉眼分级。结果：与模型组比较,蜈蚣三七纳米粉各剂量均能明显改善肠粘连程度(P<0.05或P<0.01),均可明显降低血清TNF-α、IL-1β和IL-6水平(P<0.05或P<0.01)。结论：蜈蚣三七纳米粉可抑制炎性反应因子的过度表达,减轻炎性反应,有效预防术后肠粘连的发生。 |
| English Summary: |
| To investigate the anti-inflammatory mechanism of nano-powder of Wugongsanqi (NW), the rhizome of Anemone flaccid, on postoperative intestinal adhesion in rats. Methods： Fifty SD rats were subjected to operation with Ellis' method for establishing intestinal adhesion models, then randomly divided into 5 groups (n=10), namely model, positive (Dexamethasone, i.m. 10 mg/kg), NW high, medium and low dose group (p.o. 450, 225 and 112 mg/kg, respectively). Another ten normal rats were selected as control group. Drugs were given 3 days before the operation until 7 days after the operation. The intestinal adhesion was graded and the levels of TNF-α, IL-1β and IL-6 in serum were evaluated by ELISA. Results： NW evidently reduced the severity of postoperative adhesion (P<0.05 or P<0.01), and significantly inhibited the levels of TNF-α, IL-1β and IL-6 in serum (P<0.05 or P<0.01), compared with those of the model group. Conclusion： NW could effectively prevent the formation of postoperative intestinal adhesion by inhibiting the expression of inflammatory cytokines and improving the inflammatory response. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
