To investigate the anti-inflammatory mechanism of nano-powder of Wugongsanqi (NW), the rhizome of Anemone flaccid, on postoperative intestinal adhesion in rats. Methods： Fifty SD rats were subjected to operation with Ellis' method for establishing intestinal adhesion models, then randomly divided into 5 groups (n=10), namely model, positive (Dexamethasone, i.m. 10 mg/kg), NW high, medium and low dose group (p.o. 450, 225 and 112 mg/kg, respectively). Another ten normal rats were selected as control group. Drugs were given 3 days before the operation until 7 days after the operation. The intestinal adhesion was graded and the levels of TNF-α, IL-1β and IL-6 in serum were evaluated by ELISA. Results： NW evidently reduced the severity of postoperative adhesion (P<0.05 or P<0.01), and significantly inhibited the levels of TNF-α, IL-1β and IL-6 in serum (P<0.05 or P<0.01), compared with those of the model group. Conclusion： NW could effectively prevent the formation of postoperative intestinal adhesion by inhibiting the expression of inflammatory cytokines and improving the inflammatory response.