To explore the relations between the twisting-twirling reinforcing-reducing manipulation of acupuncture and the twisting-twirling direction.Methods：Eighty Wistar rats were randomly divided into 7 groups, including a blank group (group A), model group (group B), acupuncture group (group C), right-hand-forward twisting group (group D), right-hand-backward twisting group (group E), left-hand-forward twisting group (group F) and left-hand-backward twisting group (group G). All the rats were made into blood-deficiency models by tail-bleeding and food restriction. Acupuncture was given every other day with 10 sessions totally, and the levels of red blood cell (RBC), hemoglobin (Hb), immature reticulocyte fraction(IRF) were measured at different time periods.Results：One day before the treatment, the RBC and Hb of all the groups, except the blank group (A group), decreased significantly (P<0.01), while the IRF increased obviously (P<0.01). On the 7th day of the treatment, the RBC and Hb of each group were measured and compared respectively, and the results were as follows：(a) group B was the lowest (P<0.05); (b) no statistical differences (P>0.05) were found among the group C, group D and group F; (c) no statistical differences (P>0.05) were observed among the group A, group E and group G; (d) The RBC and Hb of the first three groups were obviously higher than those of the latter three groups (P<0.05). Besides no significant IRF differences (P>0.05) were found among all the treatment groups. No statistical differences (P>0.05) were observed among all the groups on the 14th and 21th day of treatments.Conclusion：There exists no essential relation between the twisting-twirling reinforcing-reducing manipulation and the right or left twisting-twirling direction. Moreover, thumb forward twisting is “reinforcing method”, while thumb backward twisting is “reducing method”, no matter using the right or the left hand.