| 引用本文:武爱玲1 赵新杰2.HPLC-ELSD测定黄芪生络复康丸中黄芪甲苷的含量[J].世界中医药,2014,9(05):. |
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| HPLC-ELSD测定黄芪生络复康丸中黄芪甲苷的含量 |
| HPLC-ELSD Determination of Astragaloside in Huangqi Shengluo Fukang Pill |
| 投稿时间：2013-05-29 |
| DOI：10.3969/j.issn.1673-7202.2014.05.031 |
| 中文关键词: HPLC-ELSD 黄芪生络复康丸 黄芪甲苷 含量测定 |
| English Keywords:HPLC-ELSD Huangqi Shengluo Fukang Pill Astragaloside Determination |
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| 中文摘要: |
| 目的：采用HPLC-ELSD法建立黄芪生络复康丸中黄芪甲苷的含量测定方法。方法：色谱柱为Diamonsic C18（4.6 mm×250 mm，5 μm），流动相为乙腈-水（35∶65）为流动相，流速为1.0 mL·min-1，柱温为40 ℃。ELSD检测器，雾化器温度80 ℃，蒸发器温度100 ℃，载气流速1.5 mL·min-1。结果：黄芪甲苷进样量在1.62～8.10 μg时线性关系良好，r=0.999 9，平均加样回收率为99.08%（RSD=1.63%）。结论：本方法简便、准确、专属性强，可用于本品的质量控制。 |
| English Summary: |
| To establish a content determination method of Astragaloside in Huangqi Shengluo Fukang Pill by HPLC-ELSD.Methods： The chromatographic Column Diamonsic C18（4.6mm×250mm，5μm）was set. The mobile phase consisted of acetonitrile and water (35：65)， and the flow rate was 1.0mL·min-1. The column temperature was 40℃，and the content was determined with evaporate light scattering detector (ELSD). The temperature of spray chamber was 80℃ and the temperature of evaporator was 100℃.The gas flow rate of parameter of ELSD was 1.5mL·min-1.Results： The linear range of Astragaloside was within 1.62 to 8.10μg (r=0.9999).The average recovery rate was 99.08%（RSD=1.63%. Conclusion： The established method is convenient and accurate and highly specific， and it can be used for the quality control of Huangqi Shengluo Fukang Pill. |
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