| 引用本文:张琳琳1 周 震1 李 程2 张玉莲1.益肾化浊方对SAMP8小鼠NEI网络相关指标的影响[J].世界中医药,2014,9(06):. |
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| 益肾化浊方对SAMP8小鼠NEI网络相关指标的影响 |
| The Effects of Yishen Huazhuo Formula on Neuro-endocrino-immune Network in Rapid Aging Dementia Mice (SAMP8 mice) |
| 投稿时间：2014-04-28 |
| DOI：10.3969/j.issn.1673-7202.2014.06.002 |
| 中文关键词: 益肾化浊方 老年性痴呆 NEI网络 乙酰胆碱 雌二醇 促肾上腺皮质激素 白细胞介素-2 |
| English Keywords:Yishen Huazhuo Fang Alzheimers disease Neuro-endocrino-immune network Acetylcholine Estradiol Adrenocorticotropic hormone Interleukin-2 |
| 基金项目:国家重点基础研究发展计划（973计划）（编号：2010CB530405） |
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| 中文摘要: |
| 目的：观察益肾化浊方对SAMP8小鼠神经-内分泌-免疫网络（NEI网络）相关指标水平的作用，为益肾化浊方防治老年性痴呆提供实验信息和科学依据。方法：根据月龄（6、9、12月）不同共选择90只健康雄性SMAP8小鼠，各月龄小鼠分别为30只，然后同一月龄随机分为安慰剂组、安理申组和益肾化浊方组，每组10只。分别给予安理申、益肾化浊方及生理盐水灌胃，每日1次，连续灌胃4周。4周后应用酶联免疫吸附实验（ELISA）检测血清中乙酰胆碱（Ach）、雌二醇、促肾上腺皮质激素（ACTH）和白细胞介素-2（IL-2）的含量。结果：1）相同月龄与安慰剂组相比，9、12月龄小鼠安理申组与益肾化浊方组对Ach水平均有上调作用，具有统计学意义（P<0.05）。2）相同月龄与安慰剂组相比，9、12月龄小鼠益肾化浊方组对雌二醇和ACTH水平也有上调作用，对IL-2水平有下调作用，具有统计学意义（P<0.05）。3）与安慰剂组相比，9月龄小鼠益肾化浊方组Ach、雌二醇、ACTH和IL-2均有变化（P≤0.001）;与安慰剂组相比，12月龄小鼠益肾化浊方组Ach、雌二醇、ACTH和IL-2均有变化（P>0.001）。结论：益肾化浊方可通过整体多靶点调节NEI网络相关指标水平，达到治疗AD的目的。除此之外，早期防治对NEI网络稳态的恢复效果更明显。 |
| English Summary: |
| To study the change and the effect of Yishen Huazhuo formula（YSHZF） on neuro-endocrino-immune (NEI) network related indexes in rapid aging dementia mice (SAMP8 mice，so as to provide experimental evidence for preventing and treating senile dementia effect of YSHZF. Methods： A total of 90 healthy male SMAP8 mice were enrolled，which were divided into 3 groups with 30 mice per group according to age (6，9，12 months).Then the same months old rats were randomly divided into the placebo group，the Anlishen（Donepezil HCL）group and the YSHZF group，10 mice in each one. The different groups were intragastrically administered with Donepezil HCL，YSHZF or saline，1 time a day，for 4 weeks. Acetylcholine（Ach），estradiol，adrenocorticotropic hormone（ACTH）and interleukin-2（IL-2）were measured with enzyme-linked immunosorbent assay（ELISA）in all groups after 4 weeks. Results： 1）Comparing the same month old mice（9，12months），the content of Ach in the Donepezil HCL group and the YSHZF group were higher than that of the placebo group （P<0.05）. 2） Comparing the same month old mice（9，12 months），the content of estradiol and ACTH in the YSHZF group were higher than that of the placebo group while the content of IL-2 were lower（P<0.05）.（3）Comparing the same month old mice（9 month），the content of Ach，estradiol，ACTH and IL-2 were changed（P≤0.001），while in the same month（12 month）the content of Ach，estradiol，ACTH and IL-2 were also changed（P>0.001）. Conclusion：YSHZF can rectify the disorder of neuro-endocrino-immune (NEI) network so as to produce a preventing and treating effect on Alzheimer Disease from many target spots. In addition，the effect of the earlier treatment is significantly obvious. |
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