To observe the antioxidation effect of catgut embedding therapy on skin of aged rats. Methods： Thirty female wistar rats were randomly divided into control group，aged model group and catgut embedding treatment group. The rats in catgut embedding treatment group were once embedded with catgut in the back subcutaneous area; the rats in aged model group and catgut embedding treatment group were treated with D-galactose intraperitoneal injection for 100 days; rats in blank control group were not treated. One hundred days later，skins of rats were drawn back to detect skin tissue superoxide dismutase (SOD) activity，malondialdehyde (MDA) content and GHS-Px activity. Results： Compared with the control group，SOD，GHS-Px activity of skin tissue in aged model group were significantly lower (P<0.01)，and MDA levels were significantly increased (P<0.01)，but the change in catgut embedding treatment group was not significant (P>0.05). Conclusion： Catgut embedding therapy on aged rats has significant antioxidant skin effect and could antagonize aging skin.