To explore regularity of the active components alignments (ACA)through determination of the ACA in inner Mongolia wild licorice single herb，herb-herb and prescription. Methods： The ACA were determined by reversed phase high-performance liquid chromatography (RP-HPLC) in single herb，herb-herb and prescription. Results： The contents of liquiritin-liquiritigenin-glycyrrhizin acid-Isoliquiritin in single liquorice herb were（12.14±0.31），（3.25±0.19），（38.75±1.77），（l1.4±0.25; in herb-herb (honey-fried licorice-ginseng) were（2.87±0.04），（0.74±0.02），（6.42±0.18），（0.37±0.03）; in herb-herb(honey-fried licorice - bighead atractylodes rhizome ) were （5.99±0.01），（2.16±0.09），（22.4±1.8），（0.77±0.02）; in prescription were（2.99±0.03），（0.81±0.04），（10.05±0.31），（0.32±0.03）. Conclusion： There are some differences in the content of liquiritin- liquiritigenin-glycyrrhizic acid-Isoliquiritinin in single herb，herb-herb and prescription，which provides scientific basis and a new way for the establishment of improving the quality of licorice.