| 引用本文:宋添添1 牟 瑛1 张桂英2.白桦脂醇的体内抗肿瘤作用及机制[J].世界中医药,2014,9(07):. |
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| 白桦脂醇的体内抗肿瘤作用及机制 |
| Anti-tumor Effects of Betulin and Its Mechanism in Vivo |
| 投稿时间：2013-12-11 |
| DOI：10.3969/j.issn.1673-7202.2014.07.025 |
| 中文关键词: 白桦脂醇 抑瘤效应 MTT 小鼠 免疫细胞 |
| English Keywords:Betulin Anti-tumor MTT Mice Immune cells |
| 基金项目:吉林省科技厅资助项目（编号：201105020） |
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| 中文摘要: |
| 目的：研究白桦酯醇的体内抗肿瘤作用及机制。方法：采用小鼠肝癌细胞（H22）建立荷瘤小鼠动物模型，白桦脂醇灌胃10 d后，计算抑瘤率、胸腺指数、脾指数；MTT法测定小鼠T淋巴细胞转化功能及NK细胞杀伤活性。结果：白桦脂醇三个剂量组、阳性对照组瘤重均减轻，与阴性对照组比较差异有统计学意义(P<0.05)，中剂量组抑瘤率最高；三个剂量组脾指数与阳性对照组比较差异有统计学意义(P<0.05)，中、高剂量组与阴性对照组比较差异亦有统计学意义(P<0.05)；中剂量组胸腺指数与阴性、阳性对照组比较差异有统计学意义(P<0.05)；中剂量组T淋巴细胞转化率与阴性对照组和阳性对照组比较差异有统计学意义(P<0.05)；低剂量组在效靶比为25∶1时，NK细胞杀伤活性高于阳性对照组(P<0.05)，在效靶比为100∶1，杀伤活性高于阴性对照组(P<0.05)；中剂量组在效靶比为25∶1、50∶1、100∶1时，NK细胞杀伤活性均同时高于阴性对照组和阳性对照组(P<0.05)；高剂量组在效靶比为25∶1时，NK细胞杀伤活性高于阴性对照组和阳性对照组(P<0.05)。结论：白桦脂醇通过刺激激活免疫器官，提高T淋巴细胞转化功能、提高NK细胞杀伤活性来抑制肿瘤细胞增殖。 |
| English Summary: |
| To investigate the anti-tumor activity of betulin in vivo and it’s mechanism. Methods： The study built tumor bearing mice model by injecting H22 tumor cells on the mice's left inguinal region. The mice were given intragastric administration of betulin for ten days. Inhibition ratio， thymus index and spleen index were calculated after that. Then betulin's effects on T lymphocyte transformation， NK activity were detected using MTT method. Results： Tumor weight of three betulin groups and positive control group decreased compared with that of negative control group (P<0.05)， especially that of the middle dose group. Spleen index in betulin group had significant difference compared with positive control group (P<0.05). Middle dose and high dose group had significant difference from negative control group(P<0.05). Thymous index in middle dose group had significant difference compared with negative control and positive control group (P<0.05). Tlymphocyte transformation rate of middle dose group had significant difference compared with negative control and positive control group(P<0.05). When effect cell/target cell was 25， NK activity in low dose group was higher than positive control group(P<0.05)， and when effect cell/target cell was 100， it was higher than negative control group(P<0.05)； NK activity in middle dose group was higher than negative control and positive control group， when effect cell/target cell was 25 or 50 or 100 (P<0.05)； NK activity in high dose group was higher than negative control and positive control group when effect cell/target cell was 25. Conclusion： Betulin can inhibit the proliferation of tumor cell by promoting T lymphocyte transformation and enhancing NK activity. |
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