| 引用本文:刘 声1 刘晓燕2 郭霞珍2.从肺肠上皮组织细胞变化分析肺与大肠相表里的内涵[J].世界中医药,2014,9(08):. |
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| 从肺肠上皮组织细胞变化分析肺与大肠相表里的内涵 |
| Analysis of the Connotation of Interior-exteriorly Relation of the Lung and Large Intestine from Changes of the Epithelial Cells |
| 投稿时间：2013-11-18 |
| DOI：10.3969/j.issn.1673-7202.2014.08.026 |
| 中文关键词: 胎肺 细胞增殖 细胞凋亡 |
| English Keywords:Fetal lung Cell proliferation Cell apoptosis |
| 基金项目:国家自然科学基金项目基于“肺与大肠相表里”理论的肺肠同源发生的分子生物学机制研究(编号：81273614);国家973重点基础研究发展计划项目“‘肺与大肠相表里’脏腑相关理论的应用基础研究”（编号：2009CB522700） |
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| 中文摘要: |
| 目的：研究并比较人胚胎发育不同时期肺与肠上皮细胞形态特征，增殖与凋亡生物学特性，以期能为肺与大肠组织发生上的同源性提供例证，并能为中医理论中肺与大肠相互关系提供参考。方法：胚胎发育不同时期肺与肠组织石蜡包埋，HE染色，观察上皮组织及细胞形态；流式细胞术检测人胚胎不同时期肺与肠上皮细胞的增殖与凋亡，并进行分析。结果：胚胎早期（9～16周龄），肺与肠在上皮组织及细胞形态一致，上皮细胞增殖、凋亡的生物学特性无统计学意义（P>0.05）。胚胎中期(17～23周)、胚胎晚期(24周～出生)肺与肠在上皮组织及细胞形态不一致，上皮细胞增殖、凋亡的生物学特性有统计学意义（P<0.05）。结论：胚胎早期（9～16周龄）可以为“肺”与“空肠、回肠、结肠”同源发生提供一定的依据。另外，“肺与大肠相表里”的成体肺肠相关虽主要是功能之间的相互联属，但可能与其原始的同源性相关。 |
| English Summary: |
| To study and compare the human embryonic cell morphology characteristics, biological characteristics of proliferation and apoptosis of pulmonary and intestinal epithelial in different period of the growth, with a view to the lung and the large intestine tissue on the homology of illustration, and can provide a reference for the theory of traditional Chinese medicine in the lung and the large intestine relationship. Methods：Lung and intestinal tissues in different stages of the embryonic development were embedded in paraffin, HE staining, to observe the epithelial tissue and cell morphology; proliferation and apoptosis of human embryo lung and intestinal epithelial cells in different stages were detected by flow cytometry. Results： In the early embryonic stage (9 to 16 weeks), lung and intestinal epithelial tissue and cell morphology were consistent, no significant differences in the biological characteristics of epithelial cell proliferation and apoptosis (P>0.05). In medium embryonic stage (17 to 23 weeks), and late embryonic stage (24 weeks until born) epithelial tissue and cell morphology was not consistent, and there were significant differences in the biological characteristics of epithelial cell proliferation, apoptosis (P<0.05). Conclusion： Early embryonic stage (9 to 16 weeks) can provide a basis for "lung" and "the jejunum, ileum, colon" homology. In addition, the adult lung and intestine is mainly mutual affiliate between functions, but may be related to the original homology of the associated. |
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