| 引用本文:邢亚群1 王迪生1 龙子江2.附麻平喘方对哮喘模型大鼠的作用与机制的实验研究[J].世界中医药,2014,9(08):. |
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| 附麻平喘方对哮喘模型大鼠的作用与机制的实验研究 |
| Experimental Study on the Effect and Mechanism of the Fuma Pingchuan Prescription for Asthma in the Model Rats |
| 投稿时间：2014-03-06 |
| DOI：10.3969/j.issn.1673-7202.2014.08.028 |
| 中文关键词: 附麻平喘方 作用 机制 实验研究 |
| English Keywords:Fuma Pingchuan Prescription Effect Mechanism Experimental Study |
| 基金项目: |
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| 中文摘要: |
| 目的：实验探讨附麻平喘方对哮喘模型大鼠的影响并探讨其作用机制。方法：选用SPF级清洁雄性大鼠60只，采用注射卵蛋白(OVA)与氢氧化铝致敏、OVA雾化吸入激发来复制哮喘动物模型，造模后将其随机分为5组：模型组(等容量蒸馏水)、附麻防喘片组(377.5 mg/kg)、附麻平喘方高剂量组(49.68 g/kg)、附麻平喘方中剂量组(24.84 g/kg)、附麻平喘方低剂量组(12.42 g/kg)，每组10只。并于致敏2周后开始灌胃给药8周。末次给药后，采用ELISA法检测血浆ACTH，血清CORT、IL-4、IFN-γ及IgE的含量;取肺脏，一侧做HE染色，另一侧采用免疫组化法检测肺组织内β–AR的含量。结果：附麻平喘方能有效缓解模型大鼠哮喘的症状，减轻肺组织病变程度，同时显著升高血清CORT，血浆ACTH，肺组织β–AR的水平。结论：附麻平喘方可改善哮喘模型大鼠的症状，其机制可能与改善HPA轴及免疫功能有关。 |
| English Summary: |
| To examine the effect of the Fuma Pingchuan Prescription for Asthma in Experimental Rats and to study its mechanism.Methods：Sixty Clean male rats of SPF.Except the blank control group，all the rats were injected with ovalbumin(OVA) and aluminum hydroxide sensitization，OVA inhalation excited to replicate animal models of asthma.The experimental rats were divided into five groups：the model group(such as volume of distilled water)，positive compare group(377.5 mg/kg)，Fuma Pingchuan Prescription high dose group(49.68 g/kg)，middle dose group(24.84 g/kg) and low dose group(12.42 g/kg).Each group consists of ten rats.The rats started to receive intragastric administration 2 weeks after sensitization，1 dose each day in 8 weeks.After the last administration detected by ELISA in plasma ACTH，serum CORT，IL-4、IFN-γ and IgE levels.Staining the tissue in one side of the lung with HE and detecting β-AR content.In the other side by immunohistochemistry.Results：Fuma Pingchuan Prescription can effectively alleviate asthma，reduce asthma symptoms and improve lung tissue morphology，while significantly elevated serum CORT，plasma ACTH，lung tissue β-AR levels.Conclusion：Fuma Pingchuan Prescription can improve asthma symptoms of model rats，which may be related to the improved functions of HPA axis and immune. |
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