To investigate the low-frequency sound waves of somatosensory music (16 - 160Hz) on microcirculation of heart and small intestine meridian of healthy human, and biophysical mechanisms of somatosensory music treatment in aspects of meridians and collatorals.Methods：Low-frequency sound waves of somatosensory music was played on the pathway of heart and small intestine meridians in 30 healthy people, at the same time the laser Doppler was measuring the meridian acupoints of microcirculation, and statistical analysis of experimental data was conducted.Results：The different frequencies of low frequency acoustic wave had different effects on heart and small intestine; the same frequency of waves had similar effects on different acupoints of the same main and collateral channel. The F1 (43.65Hz) had the most significant impact on the Heart Sutra, and F2 (87.31Hz) had the most significant influence on the small intestine meridian.Conclusion：The heart and small intestine have selective absorption effect on low-frequency sound waves, specific frequency sound waves can cause heart and small intestine by resonance. Resonance frequency channel was F1 (43.65Hz), and resonance frequency of small intestine was F2 (87.31Hz).