世界中医药

作者	单位
周珂1 左和宁1 陈北阳2 蔡光先3	1 湖南中医药大学第一附属医院，长沙，410007； 2 湖南中医药大学，长沙，410007； 3 湖南省中医药研究院，长沙，410006

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中文摘要:

目的：观察加味黑地黄汤超微饮片对单侧输尿管结扎（Unilateral Ureteral Obstruction，UUO）大鼠肾脏纤维化的影响。方法：采用单侧输尿管结扎法复制单侧输尿管梗阻致大鼠肾间质纤维化模型，将其随机均分为空白组、假手术组、模型对照组、超微饮片组、尿毒清颗粒组，每组10只。加味黑地黄汤超微饮片组和尿毒清颗粒剂组分别予加味黑地黄汤超微饮片和尿毒清颗粒剂溶于4 mL蒸馏水灌胃，空白组、假手术组和模型组均予等体积蒸馏水灌胃。在UUO术后第14 d，将各组大鼠处死，分别取肾组织以TUNEL法检测各组大鼠肾组织细胞凋亡，并检测各组大鼠血液流变学，以及血肌酐和尿素氮。结果：经过TUNEL法检测，超微组和尿毒清颗粒组对于肾间质纤维化大鼠的肾组织细胞的凋亡均有改善作用（P<0.01），且2者作用相近（P>0.05）；尿毒清颗粒剂对UUO大鼠血液流变学的改善优于超微饮片组（P<0.05）；而超微饮片组和尿毒清颗粒剂组血肌酐和尿素氮均下降，与模型组比较有统计学意义（P<0.01或P<0.05）。结论：加味黑地黄汤超微饮片能改善UUO大鼠肾组织的细胞凋亡和血液流变学指标，改善肾组织的纤维化。

English Summary:

To observe the effect of micro decoction pieces of modified Heidihuang decoction on renal fibrosis rats caused by unilateral ureteral obstruction (UUO). Methods： Rat fibrosis models were established through unilateral ureteral obstruction, then those rats were randomly divided into blank group, sham group, model control group, micro decoction pieces group, Niaoduqing group, with 10 in each group. The micro decoction pieces of modified Heidihuang decoction and Niaoduqing granules dissolved in 4ml distilled water were respectively administered intragastrically to rats in micro decoction pieces group and Niaoduqing group. Rats in the blank group, control group and model group were all gave intra-gastric administration with the same volume of distilled water. 14 days after UUO, kidney tissues of rats in each group were taken with TUNEL method to detect kidney tissue apoptosis. At the same time, Hemorheology and serum creatinine and urea nitrogen of each group rat were detected. Results： By TUNEL method, the renal tissue cell apoptosis of micro decoction pieces group and Niaoduqing group with renal interstitial fibrosis rats were improved more better than model group(P<0.01), but there was no significant difference (P>0.05).The hemorheology of Niaoduqing group improvement was better than that of micro decoction pieces group (P<0.05); the serum creatinine and urea nitrogen of micro decoction pieces group and Niaoduqing group with renal interstitial fibrosis rats were improved better than that of the model group(P<0.01 or P<0.05). Conclusion： Micro decoction pieces of modified Heidihuang decoction can relieve renal interstitial fibrosis caused by UUO in rats by improving hemorheology indexes and renal tissue cell apoptosis.

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