世界中医药

目的：建立一种测定赤芍中芍药苷含量的高效液相色谱法，测定不同产地赤芍中芍药苷的含量并分析赤芍中重金属含量及农药的残留量。方法：色谱柱：Kromasil C18柱（5 μm，4.6 mm×250 mm）；流动相：甲醇-0.05 mol/L磷酸二氢钾（30∶70）；波长：230 nm；柱温：25 ℃；流速：1.0 mL/min；进样量：10 μL。采用原子吸收分光光度法对赤芍药材中的重金属铅、砷、铜、镉、汞进行分析，同时采用气相色谱法对有机氯类农药残留量进行测定。结果：芍药苷在0.1~1.0 mg/mL范围内，浓度与峰面积的线性关系良好（R2=0.999 6），样品的平均加样回收率为98.8%，RSD为0.29%。17个不同产地的赤芍药材中芍药苷的含量为2.17%~4.17%；赤芍中检出铅、砷、铜、镉、汞的残留量分别为0.19~0.39、0.19~0.72、0.87~8.17、0.11~0.29和0.08~0.14 mg/kg；部分赤芍药材中有检出有机氯类，DHC、DDT、PCNB的残留量分别为0.021~0.063、0.039~0.121和0.024~0.050 mg/kg。结论：17个产地的赤芍药材中芍药苷的含量均符合中国药典要求；药材存在一定量的重金属和有机氯类农药残留，除1份药材DDT含量超标外，其他药材的重金属及农药残留量均符合《药用植物及制剂进出口绿色行业标准》中的限量规定，赤芍药材总体质量较好。

English Summary:

To establish a HPLC method to determine the content of paeoniflorin as well as residual pesticide and heavy metal in Radix Paeoniae Rubra in different areas. Methods： Chromatographic column was Kromasil C18 column (5 μ m, 4.6 mm×250 mm); mobile phase was methanol -0.05mol/L potassium dihydrogen phosphate (30：70); wavelength was 230 nm; column temperature was 25 ℃; flow rate was 1 mL/min; sample size was 10 L. Analysis of heavy metal lead, arsenic, copper, cadmium, mercury in Paeonia lactiflora were determined by atomic absorption spectrophotometry, and gas chromatography was used for the determination of organochlorine pesticide residues. Results： The content of paeoniflorin was in the range of 0.1 to 1.0 mg/mL, and the linear relationship between concentration and peak area (R2=0.9996) were good. The average recovery was 98.8%, and RSD was 0.29%. The content of paeoniflorin in Radix Paeoniae Rubra from 17 different areas was 2.17~4.17%; residues of arsenic, lead, copper, cadmium, mercury in Radix Paeoniae Rubra were 0.19~0.39, 0.19~0.72, 0.87~8.17, 0.11~0.29 and 0.08~0.14mg/kg; part of Paeonia lactiflora found the detection of organochlorine residues, and DHC, DDT, PCNB were respectively 0.021~0.063, 0.039~0.121 and 0.024~0.050mg/kg. Conclusion： The contents of paeoniflorin in Paeonia lactiflora in 17 areas were all in accord with the Chinese pharmacopoeial requirements; there is a certain amount of metal materials and organic chlorine pesticide residues in medicinal materials, and except 1 copy of DDT content exceeding the standard, the quantity are in line with the “medicinal plants and preparations of the green trade standard” in the provisions on limits of heavy metal and pesticide residue in other medicinal herbs. The overall quality of Paeonia lactiflora is good.

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